An Electrochemical Immunosensor Based on a Self-Assembled Monolayer Modified Electrode for Label-Free Detection of α-Synuclein

被引:35
作者
Ge, Chuang-Ye [1 ,2 ]
Rahman, Md. Mahbubur [1 ]
Zhang, Wei [1 ]
Lopa, Nasrin Siraj [1 ]
Jin, Lei [1 ]
Yoon, Sujin [1 ]
Jang, Hohyoun [3 ]
Xu, Guang-Ri [2 ]
Kim, Whangi [1 ]
机构
[1] Konkuk Univ, Dept Energy & Mat, Chungju 380701, South Korea
[2] Henan Inst Sci & Technol, Dept Chem & Chem Engn, Xinxiang 453003, Henan, Peoples R China
[3] Konkuk Univ, Dept Liberal Arts, Chungju Si 27478, South Korea
关键词
alpha-synuclein; cystamine; immunosensor; self-assembled monolayer; fluorine-doped tin oxide; FREE APTASENSOR; DISEASE; BIOMARKERS; SURFACE;
D O I
10.3390/s20030617
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
This research demonstrated the development of a simple, cost-effective, and label-free immunosensor for the detection of alpha-synuclein (alpha-Syn) based on a cystamine (CYS) self-assembled monolayer (SAM) decorated fluorine-doped tin oxide (FTO) electrode. CYS-SAM was formed onto the FTO electrode by the adsorption of CYS molecules through the head sulfur groups. The free amine (-NH2) groups at the tail of the CYS-SAM enabled the immobilization of anti-alpha-Syn-antibody, which concurrently allowed the formation of immunocomplex by covalent bonding with alpha-Syn-antigen. The variation of the concentrations of the attached alpha-Syn at the immunosensor probe induced the alternation of the current and the charge transfer resistance (R-ct) for the redox response of [Fe(CN)(6)](3-/4-), which displayed a linear dynamic range from 10 to 1000 ng/mL with a low detection limit (S/N = 3) of ca. 3.62 and 1.13 ng/mL in differential pulse voltammetry (DPV) and electrochemical impedance spectra (EIS) measurements, respectively. The immunosensor displayed good reproducibility, anti-interference ability, and good recoveries of alpha-Syn detection in diluted human serum samples. The proposed immunosensor is a promising platform to detect alpha-Syn for the early diagnose of Parkinson's disease, which can be extended for the determination of other biologically important biomarkers.
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页数:11
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共 34 条
[31]   Biomarkers in Parkinson's disease (recent update) [J].
Sharma, Sushil ;
Moon, Carolyn Seungyoun ;
Khogali, Azza ;
Haidous, Ali ;
Chabenne, Anthony ;
Ojo, Comfort ;
Jelebinkov, Miriana ;
Kurdi, Yousef ;
Ebadi, Manuchair .
NEUROCHEMISTRY INTERNATIONAL, 2013, 63 (03) :201-229
[32]   Identification and Affinity-Quantification of -Amyloid and α-Synuclein Polypeptides Using On-Line SAW-Biosensor-Mass Spectrometry [J].
Slamnoiu, Stefan ;
Vlad, Camelia ;
Stumbaum, Mihaela ;
Moise, Adrian ;
Lindner, Kathrin ;
Engel, Nicole ;
Vilanova, Mar ;
Diaz, Mireia ;
Karreman, Christiaan ;
Leist, Marcel ;
Ciossek, Thomas ;
Hengerer, Bastian ;
Vilaseca, Marta ;
Przybylski, Michael .
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2014, 25 (08) :1472-1481
[33]   Aptasensors for the selective detection of alpha-synuclein oligomer by colorimetry, surface plasmon resonance and electrochemical impedance spectroscopy [J].
Sun, Kai ;
Xia, Ning ;
Zhao, Lijuan ;
Liu, Ke ;
Hou, Wenjing ;
Liu, Lin .
SENSORS AND ACTUATORS B-CHEMICAL, 2017, 245 :87-94
[34]   A novel electrochemical aptasensor based on nontarget-induced high accumulation of methylene blue on the surface of electrode for sensing of α-synuclein oligomer [J].
Taghdisi, Seyed Mohammad ;
Danesh, Noor Mohammad ;
Nameghi, Morteza Alinezhad ;
Ramezani, Mohammad ;
Alibolandi, Mona ;
Hassanzadeh-Khayat, Mohammad ;
Emrani, Ahmad Sarreshtehdar ;
Abnous, Khalil .
BIOSENSORS & BIOELECTRONICS, 2019, 123 :14-18