Computational 3-D reconstructions by optimization for cryo-electron Microscopy

被引:0
作者
Yin, ZY [1 ]
Zheng, YL [1 ]
Doerschuk, PC [1 ]
Johnson, JE [1 ]
机构
[1] Purdue Univ, Sch Elect & Comp Engn, W Lafayette, IN 47907 USA
来源
COMPUTATIONAL IMAGING | 2003年 / 5016卷
关键词
Flock House Virus; spherical viruses; cryo electron microscopy; icosahedral symmetry; 3-D image reconstruction; expectation maximization; maximum likelihood estimation;
D O I
10.1117/12.479705
中图分类号
TP39 [计算机的应用];
学科分类号
081203 ; 0835 ;
摘要
An algorithm for the simultaneous 3-D reconstruction of several types of object, where each type of object may possibly have a rotational symmetry, from 2-D projection images, where for each image the type of object imaged, the projection orientation used to create the image, and the location of the object in the image are unknown., is described. The motivating application is the determination of the 3-D structure of small spherical viruses from cryo electron microscopy images. The algorithm is a maximum likelihood estimator which is computed by expectation maximization (EM). Due to the structure of the statistical model, the maximization step of EM c an be easily computed but the expectation step requires 5-D numerical quadrature. The computational burden of the quadratures necessitates parallel computation and three different implementations of two different types of parallelism have been developed using pthreads (for shared memory processors) and MPI (for distributed memory processors). An example applying one of the MPI implementations, running on a 32 node PC cluster, to experimental images of Flock House Virus with comparison to the x-ray crystal diffraction structure of the virus is described.
引用
收藏
页码:71 / 79
页数:9
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