CuCl2-catalyzed inexpensive, faster and ligand/additive free synthesis of isoquinolin-1(2H)-one derivatives via the coupling-cyclization strategy: Evaluation of a new class of compounds as potential PDE4 inhibitors

被引:8
作者
Thirupataiah, B. [1 ,2 ]
Mounika, Guntipally [1 ]
Reddy, Gangireddy Sujeevan [1 ,2 ]
Kumar, Jetta Sandeep [1 ,2 ]
Kapavarapu, Ravikumar [1 ]
Medishetti, Raghavender [1 ,2 ]
Mudgal, Jayesh [2 ]
Mathew, Jessy E. [2 ]
Shenoy, Gautham G. [2 ]
Rao, C. Mallikarjuna [2 ]
Chatti, Kiranam [1 ]
Parsa, Kishore V. L. [1 ]
Pal, Manojit [1 ]
机构
[1] Dr Reddys Inst Life Sci, Univ Hyderabad Campus, Hyderabad 500046, India
[2] Manipal Acad Higher Educ, Manipal Coll Pharmaceut Sci, Manipal 576104, Karnataka, India
关键词
Isoquinolin-1(2H)-one; Cu-catalyst; PDE-4; Inflammation; In silico study; C-H ACTIVATION/ANNULATION; ANTAGONISTS; ANNULATION; EFFICIENT; ACCESS;
D O I
10.1016/j.bioorg.2021.105265
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In spite of possessing a wide range of pharmacological properties the anti-inflammatory-activities of isoquinolin-1(2H)-ones were rarely known or explored earlier. PDE4 inhibitors on the other hand in addition to their usefulness in treating inflammatory diseases have been suggested to attenuate the cytokine storm in COVID-19 especially TNF-alpha. In our effort, a new class of isoquinolin-1(2H)-ones derivatives containing an aminosulfonyl moiety were designed and explored as potential inhibitors of PDE4. Accordingly, for the first time a CuCl2-catalyzed inexpensive, faster and ligand/additive free approach has been developed for the synthesis of these predesigned isoquinolin-1(2H)-one derivatives via the coupling-cyclization strategy. Thus, the CuCl2-catalyzed reaction of 2-iodobenzamides with appropriate terminal alkynes proceeded with high chemo and regioselectivity affording the desired compounds in 77-84% yield within 1-1.5 h. The methodology also afforded simpler isoquinolin-1(2H)-ones devoid of aminosulfonyl moiety showing a broader generality and scope of this approach. Several of the synthesized compounds especially 3c , 3k and 3s showed impressive inhibition (83-90%) of PDE4B when tested at 10 mu M in vitro whereas compounds devoid of aminosulfonyl moiety was found to be less active. In spite of high inhibition showed at 10 mu M these compounds did not show proper concertation dependent inhi-bition below 1 mu M that was reflected in their IC50 values e.g. 2.43 +/- 0.32, 3.26 +/- 0.24 and 3.63 +/- 0.80 mu M for 3k , 3o and 3s respectively. The anti-inflammatory potential of these compounds was indicated by their TNF-alpha inhibition (60-50% at 10 mu M). The in silico docking studies of these molecules suggested good interactions with PDE4B and selective inhibition of PDE4B by 3k over PDE4D that was supported by in vitro assay results. These observations together with the favorable ADME and safety predicted for 3k in silico not only suggested 3k as an interesting hit molecule for further studies but also reveal the first example of isoquinolin-1(2H)-one based inhibitor of PDE4B.
引用
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页数:12
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