Selective Detection of Cu+ Ions in Live Cells via Fluorescence Lifetime Imaging Microscopy

被引：62

作者：

Priessner, Martin ^{[1
]}

Summers, Peter A. ^{[1
]}

Lewis, Benjamin W. ^{[1
]}

Sastre, Magdalena ^{[2
]}

Ying, Liming ^{[3
]}

Kuimova, Marina K. ^{[1
]}

Vilar, Ramon ^{[1
]}

机构：

[1] Imperial Coll London, Dept Chem, White City Campus, London W12 0BZ, England

[2] Imperial Coll London, Dept Brain Sci, Hammersmith Campus, London W12 0NN, England

[3] Imperial Coll London, Natl Heart & Lung Inst, Mol Sci Res Hub, White City Campus, London W12 0BZ, England

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2021年 / 60卷 / 43期

基金：

英国工程与自然科学研究理事会;

关键词：

copper(I); Flim; fluorescence; lifetime; TCSPC; PLANT-CELLS; COPPER; PROBES; RECOGNITION; LYSOSOME; RELEASE; SENSOR;

D O I：

10.1002/anie.202109349

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Copper is an essential trace element in living organisms with its levels and localisation being carefully managed by the cellular machinery. However, if misregulated, deficiency or excess of copper ions can lead to several diseases. Therefore, it is important to have reliable methods to detect, monitor and visualise this metal in cells. Herein we report a new optical probe based on BODIPY, which shows a switch-on in its fluorescence intensity upon binding to copper(I), but not in the presence of high concentration of other physiologically relevant metal ions. More interestingly, binding to copper(I) leads to significant changes in the fluorescence lifetime of the new probe, which can be used to visualize copper(I) pools in lysosomes of live cells via fluorescence lifetime imaging microscopy (FLIM).

引用

页码：23148 / 23153

页数：6

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