An improved method of protein localization in artworks through SERS nanotag-complexed antibodies

被引:43
作者
Arslanoglu, Julie [1 ]
Zaleski, Stephanie [2 ]
Loike, John [3 ]
机构
[1] Metropolitan Museum Art, New York, NY 10028 USA
[2] Columbia Univ Barnard Coll, New York, NY 10027 USA
[3] Columbia Univ, Coll Phys & Surg, Dept Physiol & Cellular Biophys, New York, NY 10032 USA
关键词
ELISA; SERS; Antibody; Nanotag; Localization; Protein; Paint cross-section; ENHANCED RAMAN-SCATTERING; WORKS-OF-ART; CROSS-SECTIONS; FTIR CHARACTERIZATION; NANOPARTICLES; SPECTROSCOPY; LABELS; DOTS; TAGS;
D O I
10.1007/s00216-010-4378-0
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
There are several analytical techniques currently in use in conservation science to identify proteins in artworks. However, as is often the case, the determination of the exact location of a protein in a complex layer structure is challenging due to difficulty in separating layers. Localization of the protein in a cross-section has been demonstrated through attenuated total reflectance-Fourier transform infrared mapping and imaging as well as chemiluminescent and fluorescent-labeled antibodies; however, these techniques either require expensive instrumental setups or produce results that can be challenging to interpret. This paper will present research using surface-enhanced Raman scattering (SERS) nanotags complexed to secondary antibodies in conjunction with primary antibodies for the localization of ovalbumin, collagen, and casein in cross-sections from replicas and artworks containing avian egg, animal glue, or casein binders. The advantages of this technique over the others are (1) the detection method is a Raman microscope, equipment found in several museum laboratories; (2) the distinctive SERS signal from the nanotag increases the detection limit of the protein and decreases the interference from other colorants present in the cross-section layers; and finally, (3) the large (120 nm) SERS-labeled antibodies do not appear to penetrate into the cross-section, eliminating the risk of spurious signal and misidentification. Any agglomerations due to surface texture are clearly visible under normal illumination and can be avoided easily during analysis or removed with a light polish. This technique not only allows protein localization in multilayered samples while preserving the stratigraphic information but also retains the protein specificity of the antibody approach.
引用
收藏
页码:2997 / 3010
页数:14
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