Recurrent aphthous stomatitis (RAS) is the most common disease affecting oral mucosae. Etiology is unknown, but several factors have been implicated, all of which influence the composition of microbiota residing on oral mucosae, which in turn modulates immunity and thereby affects disease progression. Although no individual pathogens have been conclusively shown to be causative agents of RAS, imbalanced composition of the oral microbiota may play a key role. In this study, we sought to determine composition profiles of bacterial microbiota in the oral mucosa associated with RAS. Using high-throughput 16S rRNA gene sequencing, we characterized the most abundant bacterial populations residing on healthy and ulcerated mucosae in patients with RAS (recruited using highly stringent criteria) and no associated medical conditions; we also compared these to the bacterial microbiota of healthy controls (HCs). Phylum-level diversity comparisons revealed decreased Firmicutes and increased Proteobacteria in ulcerated sites, as compared with healthy sites in RAS patients, and no differences between RAS patients with healthy sites and HCs. Genus-level analysis demonstrated higher abundance of total Bacteroidales in RAS patients with healthy sites over HCs. Porphyromonadaceae comprising species associated with periodontal disease and Veillonellaceae predominated in ulcerated sites over HCs, while no quantitative differences of these families were observed between healthy sites in RAS patients and HCs. Streptococcaceae comprising species associated with oral health predominated in HCs over ulcerated sites but not in HCs over healthy sites in RAS patients. This study demonstrates that mucosal microbiome changes in patients with idiopathic RASnamely, increased Bacteroidales species in mucosae of RAS patients not affected by active ulceration. While these changes suggest a microbial role in initiation of RAS, this study does not provide data on causality. Within this limitation, the study contributes to the understanding of the potential role of mucosal microbiome changes in oral mucosal disease.
机构:
Masaryk Univ, Fac Med, Dept Pathophysiol, Brno, Czech RepublicMasaryk Univ, Fac Med, Dept Pathophysiol, Brno, Czech Republic
Slezakova, Simona
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机构:
Borilova Linhartova, Petra
Bartova, Jirina
论文数: 0引用数: 0
h-index: 0
机构:
Charles Univ Prague, Gen Univ Hosp, Fac Med 1, Dept Stomatol, Prague, Czech RepublicMasaryk Univ, Fac Med, Dept Pathophysiol, Brno, Czech Republic
Bartova, Jirina
Petanova, Jitka
论文数: 0引用数: 0
h-index: 0
机构:
Charles Univ Prague, Gen Univ Hosp, Fac Med 1, Dept Immunol & Microbiol, Prague, Czech RepublicMasaryk Univ, Fac Med, Dept Pathophysiol, Brno, Czech Republic
Petanova, Jitka
Kuklinek, Pavel
论文数: 0引用数: 0
h-index: 0
机构:
Masaryk Univ, St Annes Fac Hosp, Fac Med, Dept Clin Immunol & Allergol, Brno, Czech RepublicMasaryk Univ, Fac Med, Dept Pathophysiol, Brno, Czech Republic
Kuklinek, Pavel
Fassmann, Antonin
论文数: 0引用数: 0
h-index: 0
机构:
Masaryk Univ, St Annes Fac Hosp, Fac Med, Clin Stomatol, Brno, Czech RepublicMasaryk Univ, Fac Med, Dept Pathophysiol, Brno, Czech Republic
Fassmann, Antonin
Dusek, Ladislav
论文数: 0引用数: 0
h-index: 0
机构:
Masaryk Univ, Fac Med, Inst Biostat & Anal, Brno, Czech RepublicMasaryk Univ, Fac Med, Dept Pathophysiol, Brno, Czech Republic
机构:Univ Fed Sao Paulo, Dept Otorhinolaryngol Head & Neck Surg, Stomatol Div, Sao Paulo, Brazil
Milanez Morgado de Abreu, Marilda Aparecida
Watashi Hirata, Cleonice Hitomi
论文数: 0引用数: 0
h-index: 0
机构:
Univ Fed Sao Paulo, Dept Otorhinolaryngol Head & Neck Surg, Stomatol Div, Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Otorhinolaryngol Head & Neck Surg, Stomatol Div, Sao Paulo, Brazil
Watashi Hirata, Cleonice Hitomi
Neto Pimentel, Dalva Regina
论文数: 0引用数: 0
h-index: 0
机构:
Univ Fed Sao Paulo, Dept Otorhinolaryngol Head & Neck Surg, Stomatol Div, Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Otorhinolaryngol Head & Neck Surg, Stomatol Div, Sao Paulo, Brazil
Neto Pimentel, Dalva Regina
Maurice Weckx, Luc Louis
论文数: 0引用数: 0
h-index: 0
机构:
Univ Fed Sao Paulo, Dept Otorhinolaryngol Head & Neck Surg, Stomatol Div, Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Otorhinolaryngol Head & Neck Surg, Stomatol Div, Sao Paulo, Brazil
Maurice Weckx, Luc Louis
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY,
2009,
108
(05):
: 714
-
721
机构:
Queen Mary Univ London, Barts & London Sch Med & Dent, Inst Dent, London E1 2AD, EnglandQueen Mary Univ London, Barts & London Sch Med & Dent, Inst Dent, London E1 2AD, England
Tappuni, Anwar R.
Kovacevic, Tatjana
论文数: 0引用数: 0
h-index: 0
机构:
Kings Coll London, Inst Dent, Guys Hosp, London, EnglandQueen Mary Univ London, Barts & London Sch Med & Dent, Inst Dent, London E1 2AD, England
Kovacevic, Tatjana
Shirlaw, Penelope J.
论文数: 0引用数: 0
h-index: 0
机构:
Kings Coll London, Inst Dent, Guys Hosp, London, EnglandQueen Mary Univ London, Barts & London Sch Med & Dent, Inst Dent, London E1 2AD, England
Shirlaw, Penelope J.
Challacombe, Stephen J.
论文数: 0引用数: 0
h-index: 0
机构:
Kings Coll London, Inst Dent, Guys Hosp, London, EnglandQueen Mary Univ London, Barts & London Sch Med & Dent, Inst Dent, London E1 2AD, England
机构:
Wake Forest Sch Med, Dept Med, Sect Pulm Crit Care Allergy & Clin Immunol, Med Ctr Blvd,Watlington Tower,Second Floor, Winston Salem, NC 27157 USA
Wake Baptist Hosp, Med Ctr Blvd,Watlington Tower,Second Floor, Winston Salem, NC 27157 USA
WG Bill Hefner VA Med Ctr, Salisbury, NC USANova Southeastern Univ, Larkin Hosp, Dept Med, Miami, FL USA
机构:
Univ Tehran Med Sci, Sch Dent, Dent Res Ctr, Tehran, IranUniv Tehran Med Sci, Sch Dent, Dent Res Ctr, Tehran, Iran
Najafi, Shamsolmoulouk
Mohammadzadeh, Mahsa
论文数: 0引用数: 0
h-index: 0
机构:
Islamic Azad Univ, Dent Branch, Dept Orthodont, Tehran, IranUniv Tehran Med Sci, Sch Dent, Dent Res Ctr, Tehran, Iran
Mohammadzadeh, Mahsa
Bidoki, Alireza Zare
论文数: 0引用数: 0
h-index: 0
机构:
Fasa Univ Med Sci, Noncommunicable Dis Res Ctr, Fasa, Iran
Univ Tehran Med Sci, Thrombosis Hemostasis Res Ctr, Tehran, IranUniv Tehran Med Sci, Sch Dent, Dent Res Ctr, Tehran, Iran
Bidoki, Alireza Zare
Meighani, Ghasem
论文数: 0引用数: 0
h-index: 0
机构:
Univ Tehran Med Sci, Sch Dent, Dept Pediat, Tehran, IranUniv Tehran Med Sci, Sch Dent, Dent Res Ctr, Tehran, Iran
Meighani, Ghasem
Aslani, Saeed
论文数: 0引用数: 0
h-index: 0
机构:
Univ Tehran Med Sci, Rheumatol Res Ctr, Tehran, Iran
Univ Tehran Med Sci, Sch Med, Dept Immunol, Tehran, IranUniv Tehran Med Sci, Sch Dent, Dent Res Ctr, Tehran, Iran
Aslani, Saeed
Mahmoudi, Mahdi
论文数: 0引用数: 0
h-index: 0
机构:
Univ Tehran Med Sci, Rheumatol Res Ctr, Tehran, IranUniv Tehran Med Sci, Sch Dent, Dent Res Ctr, Tehran, Iran
Mahmoudi, Mahdi
Rezaei, Nima
论文数: 0引用数: 0
h-index: 0
机构:
Univ Tehran Med Sci, Res Ctr Immunodeficiencies, Childrens Med Ctr, Tehran, Iran
Universal Sci Educ & Res Network, Tehran, IranUniv Tehran Med Sci, Sch Dent, Dent Res Ctr, Tehran, Iran
机构:
Masaryk Univ, Fac Med, Dept Pathophysiol, Brno, Czech RepublicMasaryk Univ, Fac Med, Dept Pathophysiol, Brno, Czech Republic
Slezakova, Simona
论文数: 引用数:
h-index:
机构:
Borilova Linhartova, Petra
Bartova, Jirina
论文数: 0引用数: 0
h-index: 0
机构:
Charles Univ Prague, Gen Univ Hosp, Fac Med 1, Dept Stomatol, Prague, Czech RepublicMasaryk Univ, Fac Med, Dept Pathophysiol, Brno, Czech Republic
Bartova, Jirina
Petanova, Jitka
论文数: 0引用数: 0
h-index: 0
机构:
Charles Univ Prague, Gen Univ Hosp, Fac Med 1, Dept Immunol & Microbiol, Prague, Czech RepublicMasaryk Univ, Fac Med, Dept Pathophysiol, Brno, Czech Republic
Petanova, Jitka
Kuklinek, Pavel
论文数: 0引用数: 0
h-index: 0
机构:
Masaryk Univ, St Annes Fac Hosp, Fac Med, Dept Clin Immunol & Allergol, Brno, Czech RepublicMasaryk Univ, Fac Med, Dept Pathophysiol, Brno, Czech Republic
Kuklinek, Pavel
Fassmann, Antonin
论文数: 0引用数: 0
h-index: 0
机构:
Masaryk Univ, St Annes Fac Hosp, Fac Med, Clin Stomatol, Brno, Czech RepublicMasaryk Univ, Fac Med, Dept Pathophysiol, Brno, Czech Republic
Fassmann, Antonin
Dusek, Ladislav
论文数: 0引用数: 0
h-index: 0
机构:
Masaryk Univ, Fac Med, Inst Biostat & Anal, Brno, Czech RepublicMasaryk Univ, Fac Med, Dept Pathophysiol, Brno, Czech Republic
机构:Univ Fed Sao Paulo, Dept Otorhinolaryngol Head & Neck Surg, Stomatol Div, Sao Paulo, Brazil
Milanez Morgado de Abreu, Marilda Aparecida
Watashi Hirata, Cleonice Hitomi
论文数: 0引用数: 0
h-index: 0
机构:
Univ Fed Sao Paulo, Dept Otorhinolaryngol Head & Neck Surg, Stomatol Div, Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Otorhinolaryngol Head & Neck Surg, Stomatol Div, Sao Paulo, Brazil
Watashi Hirata, Cleonice Hitomi
Neto Pimentel, Dalva Regina
论文数: 0引用数: 0
h-index: 0
机构:
Univ Fed Sao Paulo, Dept Otorhinolaryngol Head & Neck Surg, Stomatol Div, Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Otorhinolaryngol Head & Neck Surg, Stomatol Div, Sao Paulo, Brazil
Neto Pimentel, Dalva Regina
Maurice Weckx, Luc Louis
论文数: 0引用数: 0
h-index: 0
机构:
Univ Fed Sao Paulo, Dept Otorhinolaryngol Head & Neck Surg, Stomatol Div, Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Otorhinolaryngol Head & Neck Surg, Stomatol Div, Sao Paulo, Brazil
Maurice Weckx, Luc Louis
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY,
2009,
108
(05):
: 714
-
721
机构:
Queen Mary Univ London, Barts & London Sch Med & Dent, Inst Dent, London E1 2AD, EnglandQueen Mary Univ London, Barts & London Sch Med & Dent, Inst Dent, London E1 2AD, England
Tappuni, Anwar R.
Kovacevic, Tatjana
论文数: 0引用数: 0
h-index: 0
机构:
Kings Coll London, Inst Dent, Guys Hosp, London, EnglandQueen Mary Univ London, Barts & London Sch Med & Dent, Inst Dent, London E1 2AD, England
Kovacevic, Tatjana
Shirlaw, Penelope J.
论文数: 0引用数: 0
h-index: 0
机构:
Kings Coll London, Inst Dent, Guys Hosp, London, EnglandQueen Mary Univ London, Barts & London Sch Med & Dent, Inst Dent, London E1 2AD, England
Shirlaw, Penelope J.
Challacombe, Stephen J.
论文数: 0引用数: 0
h-index: 0
机构:
Kings Coll London, Inst Dent, Guys Hosp, London, EnglandQueen Mary Univ London, Barts & London Sch Med & Dent, Inst Dent, London E1 2AD, England
机构:
Wake Forest Sch Med, Dept Med, Sect Pulm Crit Care Allergy & Clin Immunol, Med Ctr Blvd,Watlington Tower,Second Floor, Winston Salem, NC 27157 USA
Wake Baptist Hosp, Med Ctr Blvd,Watlington Tower,Second Floor, Winston Salem, NC 27157 USA
WG Bill Hefner VA Med Ctr, Salisbury, NC USANova Southeastern Univ, Larkin Hosp, Dept Med, Miami, FL USA
机构:
Univ Tehran Med Sci, Sch Dent, Dent Res Ctr, Tehran, IranUniv Tehran Med Sci, Sch Dent, Dent Res Ctr, Tehran, Iran
Najafi, Shamsolmoulouk
Mohammadzadeh, Mahsa
论文数: 0引用数: 0
h-index: 0
机构:
Islamic Azad Univ, Dent Branch, Dept Orthodont, Tehran, IranUniv Tehran Med Sci, Sch Dent, Dent Res Ctr, Tehran, Iran
Mohammadzadeh, Mahsa
Bidoki, Alireza Zare
论文数: 0引用数: 0
h-index: 0
机构:
Fasa Univ Med Sci, Noncommunicable Dis Res Ctr, Fasa, Iran
Univ Tehran Med Sci, Thrombosis Hemostasis Res Ctr, Tehran, IranUniv Tehran Med Sci, Sch Dent, Dent Res Ctr, Tehran, Iran
Bidoki, Alireza Zare
Meighani, Ghasem
论文数: 0引用数: 0
h-index: 0
机构:
Univ Tehran Med Sci, Sch Dent, Dept Pediat, Tehran, IranUniv Tehran Med Sci, Sch Dent, Dent Res Ctr, Tehran, Iran
Meighani, Ghasem
Aslani, Saeed
论文数: 0引用数: 0
h-index: 0
机构:
Univ Tehran Med Sci, Rheumatol Res Ctr, Tehran, Iran
Univ Tehran Med Sci, Sch Med, Dept Immunol, Tehran, IranUniv Tehran Med Sci, Sch Dent, Dent Res Ctr, Tehran, Iran
Aslani, Saeed
Mahmoudi, Mahdi
论文数: 0引用数: 0
h-index: 0
机构:
Univ Tehran Med Sci, Rheumatol Res Ctr, Tehran, IranUniv Tehran Med Sci, Sch Dent, Dent Res Ctr, Tehran, Iran
Mahmoudi, Mahdi
Rezaei, Nima
论文数: 0引用数: 0
h-index: 0
机构:
Univ Tehran Med Sci, Res Ctr Immunodeficiencies, Childrens Med Ctr, Tehran, Iran
Universal Sci Educ & Res Network, Tehran, IranUniv Tehran Med Sci, Sch Dent, Dent Res Ctr, Tehran, Iran