Interleukin-10 inhibits the down-regulation of ATP binding cassette transporter A1 by tumour necrosis factor-alpha in THP-1 macrophage-derived foam cells

被引：35
作者：
Mei, Chun-li [1 ]
Chen, Zhi-jian [1 ]
Liao, Yu-hua [1 ]
Wang, Yan-fu [1 ]
Peng, Hong-yu [1 ]
Chen, Yu [1 ]
机构：
[1] Huazhong Univ Sci & Technol, Tongji Med Coll, Union Hosp, Dept Cardiol, Wuhan 430022, Hubei Province, Peoples R China
来源：
CELL BIOLOGY INTERNATIONAL | 2007年 / 31卷 / 12期
关键词：
ATP binding cassette transporter A1; cholesterol content; tumour necrosis factor-alpha; interleukin-10; THP-1 macrophage-derived foam cells;
D O I：
10.1016/j.cellbi.2007.06.009
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
It is suggested that cholesterol efflux mediated by ATP binding cassette transporter A1 (ABCA1) plays an important role in anti-atherogenesis. However, the effects of inflammatory cytokines on ABCA1 expression and cholesterol accumulation in foam cells are little known. This study investigates the effects of tumour necrosis factor-alpha (TNF-alpha) and interleukin-10 (IL-10) on ABCA1 expression and cholesterol content in THP-1 macrophage-derived foam cells. ABCA1mRNA and protein levels were determined by RT-PCR and Western blot, respectively. The total cholesterol content in THP-1 macrophage-derived foam cells was detected by the zymochemistry method. Results revealed that TNF-alpha could increase cholesterol content by down-regulating ABCA1 expression in a time-dependent manner in THP-1 macrophage-derived foam cells, which may contribute to its pro-atherosclerotic effect. In addition IL-10 time-dependently decreased cholesterol accumulation by up-regulating ABCA1 expression and inhibited the down-regulation of ABCA1 by TNF-alpha in THP-1 macrophage-derived foam cells, which may be one of the mechanisms of IL-10 contributing to its anti-atherosclerotic action. (c) 2007 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码：1456 / 1461
页数：6
相关论文
共 30 条
  • [1] Increased atherosclerosis in hyperlipidemic mice with inactivation of ABCA1 in macrophages
    Aiello, RJ
    Brees, D
    Bourassa, PA
    Royer, L
    Lindsey, S
    Coskran, T
    Haghpassand, M
    Francone, OL
    [J]. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2002, 22 (04) : 630 - 637
  • [2] Activated transcription factor nuclear factor-kappa B is present in the atherosclerotic lesion
    Brand, K
    Page, S
    Rogler, G
    Bartsch, A
    Brandl, R
    Knuechel, R
    Page, M
    Kaltschmidt, C
    Baeuerle, PA
    Neumeier, D
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1996, 97 (07) : 1715 - 1722
  • [3] Regulation of plasma high-density lipoprotein levels by the ABCA1 transporter and the emerging role of high-density lipoprotein in the treatment of cardiovascular disease
    Brewer, HB
    Remaley, AT
    Neufeld, EB
    Basso, F
    Joyce, C
    [J]. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2004, 24 (10) : 1755 - 1760
  • [4] Interleukin-10 deficiency increases atherosclerosis, thrombosis, and low-density lipoproteins in apolipoprotein E knockout mice
    Caligiuri, G
    Rudling, M
    Ollivier, V
    Jacob, MP
    Michel, JB
    Hansson, GK
    Nicoletti, A
    [J]. MOLECULAR MEDICINE, 2003, 9 (1-2) : 10 - 17
  • [5] Increased cholesterol deposition, expression of scavenger receptors, and response to chemotactic factors in Abca1-deficient macrophages
    Francone, OL
    Royer, L
    Boucher, G
    Haghpassand, M
    Freeman, A
    Brees, D
    Aiello, RJ
    [J]. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2005, 25 (06) : 1198 - 1205
  • [6] GAMBLE W, 1978, J LIPID RES, V19, P1068
  • [7] TNFα induces ABCA1 through NF-κB in macrophages and in phagocytes ingesting apoptotic cells
    Gerbod-Giannone, MC
    Li, YK
    Holleboom, A
    Han, S
    Hsu, LC
    Tabas, I
    Tall, AR
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (09) : 3112 - 3117
  • [8] Mechanisms of disease - Inflammation, atherosclerosis, and coronary artery disease
    Hansson, GK
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 2005, 352 (16) : 1685 - 1695
  • [9] Localization of human ATP-binding cassette transporter 1 (ABC1) in normal and atherosclerotic tissues
    Lawn, RM
    Wade, DP
    Couse, TL
    Wilcox, JN
    [J]. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2001, 21 (03) : 378 - 385
  • [10] Libby P, 2002, NATURE, V420, P868, DOI [10.1038/nature01323, 10.1161/ATVBAHA.108.179705]
123