Rapid preparation of a panel of polyclonal antibodies to Drosophila segmentation proteins

被引：217
作者：
Kosman, D
Small, S
Reinitz, J
机构：
[1] CUNY Mt Sinai Sch Med, Brookdale Ctr Mol Biol, New York, NY 10029 USA
[2] NYU, Dept Biol, New York, NY 10003 USA
来源：
DEVELOPMENT GENES AND EVOLUTION | 1998年 / 208卷 / 05期
关键词：
Drosophila; gene regulation; segmentation; immunological methods;
D O I：
10.1007/s004270050184
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
We describe a method for rapidly raising a panel of high quality polyclonal antibodies from bacterially expressed proteins. Approximately 1 2/3 days of preparation is required per protein. One step that speeds up the procedure is the visualization of purified bands by precipitated sodium dodecyl sulfate (SDS). Antigenicity of the purified recombinant proteins may be increased by precipitation in double-distilled water. The results of using the serums obtained for fluorescent staining of Drosophila embryos are shown.
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收藏
页码：290 / 294
页数：5
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