Mapping Polymer Phase Diagram in Nanoliter Droplets

被引：6

作者：

Shi, Feng ^{[1
]}

Han, Zuoyan ^{[1
]}

Li, Junfang ^{[1
]}

Zheng, Bo ^{[1
]}

Wu, Chi ^{[1
,2
]}

机构：

[1] Chinese Univ Hong Kong, Dept Chem, Shatin, Hong Kong, Peoples R China

[2] Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Dept Chem Phys, Hefei 230026, Anhui, Peoples R China

来源：

MACROMOLECULES | 2011年 / 44卷 / 04期

关键词：

PROTEIN CRYSTALLIZATION CONDITIONS; MICROFLUIDIC SYSTEM; LIGHT-SCATTERING; SINGLE-CHAIN; POLY(N-ISOPROPYLACRYLAMIDE); TRANSITION; BEHAVIOR; WATER; GLOBULE;

D O I：

10.1021/ma102917u

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Microfluidic systems provide an attractive platform to address these two problems by significantly reducing the consumption of reagents and the time for reaching thermal equilibrium. The polymer concentration in each droplet was determined by using fluorescent internal marker. After precisely determining the PNIPAM concentration in each individual droplet, the phase transition of the PNIPAM aqueous solution was detected. The scattered light intensities of each individual droplet at various temperatures were measured by ImageJ (NIH). The droplets containing the PNIPAM solution were separated from the capillary wall by a thin layer of silicone oil. The droplets were about 200 μm in diameter and 550 μm in length and presented large ratio of surface area to volume (S/V). The larger S/V ratio enhanced adsorption of PNIPAM to the water/oil interface, which could change the phase transition behavior and also cause uneven distribution of the scattering light intensity across the droplets.

引用

页码：686 / 689

页数：4

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