Theabrownin suppresses in vitro osteoclastogenesis and prevents bone loss in ovariectomized rats

被引：28

作者：

Liu, Titi ^{[1
,2
,3
]}

Xiang, Zemin ^{[1
,2
,3
]}

Chen, Fei ^{[1
,2
,3
]}

Yin, Dan ^{[1
,2
,3
]}

Huang, Yewei ^{[1
,2
,3
]}

Xu, Jing ^{[1
,2
,3
]}

Hu, Lihong ^{[1
,2
,3
]}

Xu, Huanhuan ^{[1
,2
,3
]}

Wang, Xuanjun ^{[1
,2
,4
,5
]}

Sheng, Jun ^{[1
,2
,5
]}

机构：

[1] Yunnan Agr Univ, Key Lab Pu Er Tea Sci, Minist Educ, Kunming 650201, Yunnan, Peoples R China

[2] Tea Res Ctr Yunnan, Kunming 650201, Yunnan, Peoples R China

[3] Yunnan Agr Univ, Coll Food Sci & Technol, Kunming 650201, Yunnan, Peoples R China

[4] Yunnan Agr Univ, Coll Sci, Kunming 650201, Yunnan, Peoples R China

[5] State Key Lab Conservat & Utilizat Bioresources Y, Kunming 650201, Yunnan, Peoples R China

来源：

BIOMEDICINE & PHARMACOTHERAPY | 2018年 / 106卷

关键词：

Theabrownin; Osteoporosis; Osteoclastogenesis; RANKL; Bone loss; TEA POLYPHENOLS; RAW264.7; CELLS; OSTEOPOROSIS; DIFFERENTIATION; RALOXIFENE; METABOLISM; HEALTH;

D O I：

10.1016/j.biopha.2018.07.103

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Drinking tea exhibits beneficial effects on bone health and may protect against osteoporosis, particularly in postmenopausal women. Theabrownin (TB) is the main component responsible for the biological activities of Puerh tea, but whether it possesses anti-osteoporotic potential remains unknown. Here we investigated the in vitro and in vivo anti-osteoporotic effects of TB in the RAW 264.7 cell line and ovariectomized (OVX) rats, respectively. Our in vitro studies showed that TB significantly suppressed RANKL-induced osteoclastogenesis and the expression of related marker proteins, including NFATc1, TRAP, c-Fos, and cathepsin K. In vivo studies showed that TB treatment effectively ameliorated blood biochemical parameters, organ weights and organ coefficients in OVX rats. In addition, TB treatment significantly improved femoral bone mineral density (BMD) and biomechanical properties. What's more, TB treatment strikingly ameliorated bone microarchitecture in OVX rats because of increased cortical bone thickness and trabecular bone area in the femur. Our study therefore demonstrated that TB can inhibit RANKL-induced osteoclastogenesis in vitro and prevent bone loss in ovariectomized rats. Consequently, TB has a promising potential in postmenopausal osteoporosis treatment.

引用

页码：1339 / 1347

页数：9