Selective determination of homocysteine at physiological pH using nanostructured film of aminothiadiazole modified electrode

被引：10

作者：

Kalimuthu, Palraj ^{[1
]}

John, S. Abraham ^{[1
]}

机构：

[1] Gandhigram Rural Univ, Dept Chem, Gandhigram 624302, Dindigul, India

来源：

BIOELECTROCHEMISTRY | 2010年 / 79卷 / 02期

关键词：

Electropolymerization; 2-Amino-1,3,4-thiadiazole; AFM; Homocysteine; Ascorbic acid; Amperometry; ELECTROCHEMICAL DETECTION; LIQUID-CHROMATOGRAPHY; HUMAN PLASMA; THIOLS; CYSTEINE; METHIONINE; OXIDATION; DISEASE;

D O I：

10.1016/j.bioelechem.2010.02.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the selective determination of homocysteine (HCY) in the presence of one of the very important interferents, ascorbic acid (AA) using electropolymerized film of 2-amino-1,3,4-thiadiazole (ATD) modified glassy carbon electrode (GCE) at physiological pH for the first time. An atomic force microscopic image showed that the electropolymerized film of ATD (p-ATD) formed a spherical like structure with a thickness of 25 nm. This nanostructured film oxidized HCY at 0.55 V while bare GCE failed to oxidize it at physiological pH. Further, p-ATD modified electrode successfully separated the voltammetric signals of AA and HCY with a peak separation of 490 mV. The amperometric current was increased linearly from 100 to 1400 nM HCY and achieved the detection limit of 51 pM (S/N= 3). The present modified electrode showed better recoveries for spiked HCY in human blood serum samples. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：168 / 172

页数：5

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