Fibronectin in cell adhesion and migration via N-glycosylation

被引:90
作者
Hsiao, Cheng-Te [1 ,2 ]
Cheng, Hung-Wei [3 ]
Huang, Chi-Ming [3 ]
Li, Hao-Ru [3 ]
Ou, Meng-Hsin [3 ]
Huang, Jie-Rong [3 ]
Khoo, Kay-Hooi [1 ,2 ]
Yu, Helen Wenshin [4 ]
Chen, Yin-Quan [4 ]
Wang, Yang-Kao [5 ]
Chiou, Arthur [4 ,6 ]
Kuo, Jean-Cheng [3 ,4 ,7 ]
机构
[1] Natl Taiwan Univ, Inst Biochem Sci, Taipei 10617, Taiwan
[2] Acad Sinica, Inst Biol Chem, Taipei 115, Taiwan
[3] Natl Yang Ming Univ, Inst Biochem & Mol Biol, Taipei 11221, Taiwan
[4] Natl Yang Ming Univ, Biophoton & Mol Imaging Res Ctr, Taipei 11221, Taiwan
[5] Natl Cheng Kung Univ, Dept Cell Biol & Anat, Tainan 70101, Taiwan
[6] Natl Yang Ming Univ, Inst Biophoton, Taipei 11221, Taiwan
[7] Natl Yang Ming Univ, Prote Res Ctr, Taipei 11221, Taiwan
关键词
fibronectin; glycoproteomics; N-glycans; integrin signals; cell migration; HUMAN-PLASMA; EXTRACELLULAR-MATRIX; AFFINITY; ACID; IDENTIFICATION; PURIFICATION; INTEGRINS; GELATIN;
D O I
10.18632/oncotarget.19969
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Directed cell migration is an important step in effective wound healing and requires the dynamic control of the formation of cell-extracellular matrix interactions. Plasma fibronectin is an extracellular matrix glycoprotein present in blood plasma that plays crucial roles in modulating cellular adhesion and migration and thereby helping to mediate all steps of wound healing. In order to seek safe sources of plasma fibronectin for its practical use in wound dressing, we isolated fibronectin from human (homo) and porcine plasma and demonstrated that both have a similar ability as a suitable substrate for the stimulation of cell adhesion and for directing cell migration. In addition, we also defined the N-glycosylation sites and N-glycans present on homo and porcine plasma fibronectin. These N-glycosylation modifications of the plasma fibronectin synergistically support the integrin-mediated signals to bring about mediating cellular adhesion and directed cell migration. This study not only determines the important function of N-glycans in both homo and porcine plasma fibronectin-mediated cell adhesion and directed cell migration, but also reveals the potential applications of porcine plasma fibronectin if it was applied as a material for clinical wound healing and tissue repair.
引用
收藏
页码:70653 / 70668
页数:16
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