Development of resistance during antimicrobial therapy caused by insertion sequence interruption of porin genes

被引：72

作者：

Hernández-Allés, S

Benedí, VJ

Martínez-Martínez, L

Pascual, A

Aguilar, A

Tomás, JM

Albertí, S

机构：

[1] Univ Balearic Isl, Area Microbiol, Dept Biol, E-07071 Palma de Mallorca, Spain

[2] IMEDEA, UIB, CSIC, Palma de Mallorca, Spain

[3] Univ Sevilla, Dept Microbiol, Seville, Spain

[4] Univ Barcelona, Dept Microbiol, Barcelona, Spain

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1999年 / 43卷 / 04期

关键词：

D O I：

10.1128/AAC.43.4.937

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have demonstrated by using an in vitro approach that interruption of the OmpK36 porin gene by insertion sequences (ISs) is a common type of mutation that causes toss of porin expression and increased resistance to cefoxitin in Klebsiella pneumoniae. This mechanism also operates in vivo: of 13 porin-deficient cefoxitin-resistant clinical isolates of K. pneumoniae, 4 presented ISs in their ompK36 gene.

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收藏

页码：937 / 939

页数：3

相关论文

共 20 条

[1]

AGGELER R, 1987, J GEN MICROBIOL, V133, P3383

[2]

ALBERTI S, 1995, INFECT IMMUN, V63, P903

[3] Outer membrane profiles of clonally related Klebsiella pneumoniae isolates from clinical samples and activities of cephalosporins and carbapenems [J].

Ardanuy, C ;

Liñares, J ;

Domínguez, MA ;

Hernández-Allés, S ;

Benedi, VJ ;

Martínez-Martínez, L .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1998, 42 (07) :1636-1640

[4]

Ausubel F.A., 1997, CURRENT PROTOCOLS MO, DOI DOI 10.1.4

[5] SOLUBILIZATION OF CYTOPLASMIC MEMBRANE OF ESCHERICHIA-COLI BY IONIC DETERGENT SODIUM-LAURYL SARCOSINATE [J].

FILIP, C ;

FLETCHER, G ;

WULFF, JL ;

EARHART, CF .

JOURNAL OF BACTERIOLOGY, 1973, 115 (03) :717-722

[6] Porin expression in clinical isolates of Klebsiella pneumoniae [J].

Hernández-Allés, S ;

Albertí, S ;

Alvarez, D ;

Doménech-Sánchez, A ;

Martínez-Martínez, L ;

Gil, J ;

Tomás, JM ;

Benedí, VJ .

MICROBIOLOGY-UK, 1999, 145 :673-679

[7] ISOLATION OF FC3-11, A BACTERIOPHAGE SPECIFIC FOR THE KLEBSIELLA-PNEUMONIAE PORIN OMPK36, AND ITS USE FOR THE ISOLATION OF PORIN-DEFICIENT MUTANTS [J].

HERNANDEZALLES, S ;

ALBERTI, S ;

RUBIRES, X ;

MERINO, S ;

TOMAS, JM ;

BENEDI, VJ .

CANADIAN JOURNAL OF MICROBIOLOGY, 1995, 41 (4-5) :399-406

[8] INSERTION OF IS2 CREATES A NOVEL AMPC PROMOTER IN ESCHERICHIA-COLI [J].

JAURIN, B ;

NORMARK, S .

CELL, 1983, 32 (03) :809-816

[9] In vivo selection of porin-deficient mutants of Klebsiella pneumoniae with increased resistance to cefoxitin and expanded-spectrum cephalosporins [J].

MartinezMartinez, L ;

HernandezAlles, S ;

Alberti, S ;

Tomas, JM ;

Benedi, VJ ;

Jacoby, GA .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1996, 40 (02) :342-348

[10] LOSS OF OMPC PORIN IN A STRAIN OF SALMONELLA-TYPHIMURIUM CAUSES INCREASED RESISTANCE TO CEPHALOSPORINS DURING THERAPY [J].

MEDEIROS, AA ;

OBRIEN, TF ;

ROSENBERG, EY ;

NIKAIDO, H .

JOURNAL OF INFECTIOUS DISEASES, 1987, 156 (05) :751-757