Detection of common resistance genes of Gram-negative bacteria by DNA microarray assay

被引：1
作者：
Cai, Ting [1 ]
Zhang, Shun [1 ]
Li, Qiao-yun [1 ]
Zhang, Chun-xiu [1 ]
Chang, Yan-zi [1 ]
机构：
[1] Ningbo 2 Hosp, Ningbo 315010, Zhejiang, Peoples R China
来源：
AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH | 2012年 / 6卷 / 02期
关键词：
Gram-negative bacteria; resistance gene; DNA microarray;
D O I：
10.5897/AJMR11.1068
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
To design a DNA microarray for the detection of common resistance genes of Gram-negative bacteria, we collected 70 strains of Gram-negative bacilli (Klebsiella pneumoniae, Escherichia coli, Enterobacter cloacae, Pseudomonas aeruginosa, Acinetobacter baumannii), and the primers and probes were designed and synthesized according to the known resistance genes to prepare the deoxyribonucleic acid (DNA) microarray for the detection of these resistance genes. Then, hybridization and subsequent scanning were performed. The results of DNA microarray assay were compared with results from the polymerase chain reaction (PCR). Results showed that the consistency rate was 100.00% in the detection of 8 resistance genes (TEM, SHV, CTX-M, DHA, CIT, VIM, KPC and OXA-23). PCR assay revealed the resistance gene IMP in 1 strain of P. aeruginosa, which was absent in the DNA microarray assay. The DNA microarray designed in the present study has clinical value in the detection of common resistance genes of Gram-negative bacteria.
引用
收藏
页码：371 / 378
页数：8
相关论文
共 50 条
  • [41] Hydroxyl radicals dominated the reduction of antibiotic resistance genes by inactivating Gram-negative bacteria during soil electrokinetic treatment
    Li, Binxu
    Jiang, Kaiyang
    Song, Tingting
    Yan, Mengmeng
    Li, Na
    Yang, Zhenzhen
    Zhu, Changxiong
    Li, Hongna
    JOURNAL OF ENVIRONMENTAL MANAGEMENT, 2024, 370
  • [42] Correlation between Identification of β-Lactamase Resistance Genes and Antimicrobial Susceptibility Profiles in Gram-Negative Bacteria: a Laboratory Data Analysis
    Mushtaq, Ammara
    Chasan, Rachel
    Nowak, Michael D.
    Rana, Meenakshi
    Ilyas, Sahrish
    Paniz-Mondolfi, Alberto E.
    Sordillo, Emilia M.
    Patel, Gopi
    Gitman, Melissa R.
    MICROBIOLOGY SPECTRUM, 2022, 10 (02):
  • [43] Microconductometric immunosensor for label-free and sensitive detection of Gram-negative bacteria
    El Ichi, Sarra
    Leon, Fanny
    Vossier, Ludivine
    Marchandin, Helene
    Errachid, Abdelhamid
    Coste, Joliette
    Jaffrezic-Renault, Nicole
    Fournier-Wirth, Chantal
    BIOSENSORS & BIOELECTRONICS, 2014, 54 : 378 - 384
  • [44] Estrogen mimics induce genes encoding chemical efflux proteins in gram-negative bacteria
    Li, Xinhua
    Teske, Sondra
    Conroy-Ben, Otakuye
    CHEMOSPHERE, 2015, 128 : 327 - 331
  • [45] Prevalence of multidrug-resistant Gram-negative bacteria from blood cultures and rapid detection of beta-lactamase-encoding genes by multiplex PCR assay
    Zornic, Sanja
    Lukovic, Bojana
    Petrovic, Ivana
    Jencic, Aleksandra
    GERMS, 2022, 12 (04): : 434 - 443
  • [46] Bismuth Drugs Reverse Tet(X)-Conferred Tigecycline Resistance in Gram-Negative Bacteria
    Deng, Tian
    Jia, Yuqian
    Tong, Ziwen
    Shi, Jingru
    Wang, Zhiqiang
    Liu, Yuan
    MICROBIOLOGY SPECTRUM, 2022, 10 (01):
  • [47] Quorum Sensing in Gram-Negative Bacteria: Strategies to Overcome Antibiotic Resistance in Ocular Infections
    Tiwari, Sakshi
    Gidwani, Bina
    Vyas, Amber
    CURRENT MOLECULAR MEDICINE, 2024, 24 (07) : 876 - 888
  • [48] Impact of Polymyxin Resistance on Virulence and Fitness among Clinically Important Gram-Negative Bacteria
    Wang, Yuan
    Luo, Qixia
    Xiao, Tingting
    Zhu, Yunying
    Xiao, Yonghong
    ENGINEERING, 2022, 13 : 178 - 185
  • [49] Overcoming Intrinsic and Acquired Resistance Mechanisms Associated with the Cell Wall of Gram-Negative Bacteria
    Impey, Rachael E.
    Hawkins, Daniel A.
    Sutton, J. Mark
    Soares da Costa, Tatiana P.
    ANTIBIOTICS-BASEL, 2020, 9 (09): : 1 - 19
  • [50] Prevalence of Gram-negative and Gram-positive Bacteria and Antibiotic Resistance Rates at a Military Hospital in Riyadh Region
    Ahmed, Nehad Jaser
    Abdalla, Mohammad
    Alahmadi, Hadeel
    Haseeb, Abdul
    Khan, Amer Hayat
    JOURNAL OF YOUNG PHARMACISTS, 2021, 13 (04) : 392 - 395
12345