Particulate vaccines are emerging promising technologies for the creation of tunable prophylactics against a wide variety of conditions. Vesicular and solid biodegradable polymer platforms, exemplified by liposomes and polyesters, respectively, are two of the most ubiquitous platforms in vaccine delivery studies. Here we directly compared the efficacy of each in a long-term immunization study and in protection against a model bacterial antigen. Immunization with poly(lactide-co-glycolide) (PLGA) nanoparticles elicited prolonged antibody titers compared to liposomes and alum. The magnitude of the cellular immune response was also highest in mice vaccinated with PLGA, which also showed a higher frequency of effector-like memory T cell phenotype, leading to an effective clearance of intracellular bacteria. The difference in performance of these two common particulate platforms is shown not to be due to material differences but appears to be connected to the kinetics of antigen delivery. Thus, this study highlights the importance of sustained antigen release mediated by particulate platforms and its role in the long-term appearance of effector memory cellular response. (c) 2012 Elsevier Ltd. All rights reserved.
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Yale Univ, Dept Biomed Engn, New Haven, CT 06511 USAYale Univ, Dept Biomed Engn, New Haven, CT 06511 USA
Bandyopadhyay, Arunima
;
Fine, Rebecca L.
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Yale Univ, Sch Med, Dept Internal Med, New Haven, CT 06511 USA
Williams Coll, Williamstown, MA 01267 USAYale Univ, Dept Biomed Engn, New Haven, CT 06511 USA
Fine, Rebecca L.
;
Demento, Stacey
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Yale Univ, Dept Biomed Engn, New Haven, CT 06511 USAYale Univ, Dept Biomed Engn, New Haven, CT 06511 USA
Demento, Stacey
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Bockenstedt, Linda K.
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Yale Univ, Sch Med, Dept Internal Med, New Haven, CT 06511 USAYale Univ, Dept Biomed Engn, New Haven, CT 06511 USA
Bockenstedt, Linda K.
;
Fahmy, Tarek M.
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Yale Univ, Dept Biomed Engn, New Haven, CT 06511 USA
Yale Univ, Dept Chem Engn, New Haven, CT 06511 USAYale Univ, Dept Biomed Engn, New Haven, CT 06511 USA
机构:
Yale Univ, Dept Biomed Engn, New Haven, CT 06511 USAYale Univ, Dept Biomed Engn, New Haven, CT 06511 USA
Bandyopadhyay, Arunima
;
Fine, Rebecca L.
论文数: 0引用数: 0
h-index: 0
机构:
Yale Univ, Sch Med, Dept Internal Med, New Haven, CT 06511 USA
Williams Coll, Williamstown, MA 01267 USAYale Univ, Dept Biomed Engn, New Haven, CT 06511 USA
Fine, Rebecca L.
;
Demento, Stacey
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h-index: 0
机构:
Yale Univ, Dept Biomed Engn, New Haven, CT 06511 USAYale Univ, Dept Biomed Engn, New Haven, CT 06511 USA
Demento, Stacey
;
Bockenstedt, Linda K.
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h-index: 0
机构:
Yale Univ, Sch Med, Dept Internal Med, New Haven, CT 06511 USAYale Univ, Dept Biomed Engn, New Haven, CT 06511 USA
Bockenstedt, Linda K.
;
Fahmy, Tarek M.
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h-index: 0
机构:
Yale Univ, Dept Biomed Engn, New Haven, CT 06511 USA
Yale Univ, Dept Chem Engn, New Haven, CT 06511 USAYale Univ, Dept Biomed Engn, New Haven, CT 06511 USA