Neuroprotective Effects of Euonymus alatus Extract on Scopolamine-Induced Memory Deficits in Mice

被引:34
作者
Woo, Yunju [1 ,2 ]
Lim, Ji Sun [1 ,2 ]
Oh, Jisun [1 ,2 ]
Lee, Jeong Soon [3 ]
Kim, Jong-Sang [1 ,2 ]
机构
[1] Kyungpook Natl Univ, Sch Food Sci & Biotechnol BK21 Plus, Daegu 41566, South Korea
[2] Kyungpook Natl Univ, Inst Agr Sci & Technol, Daegu 41566, South Korea
[3] Forest Resources Dev Inst Gyeongsangbuk Do, Andong 36605, South Korea
关键词
Euonymus alatus; antioxidant; BDNF; cognition improvement; neuroprotective; HT22; cells; animal model; ANTIOXIDANT ACTIVITY; ALZHEIMERS-DISEASE; CHEMICAL-CONSTITUENTS; QUERCETIN; BRAIN; ACETYLCHOLINESTERASE; IMPAIRMENT; NEURONS; RUTIN; DPPH;
D O I
10.3390/antiox9050449
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Euonymus alatus is considered to elicit various beneficial effects against cancer, hyperglycemia, menstrual discomfort, diabetic complications, and detoxification. The young leaves of this plant are exploited as food and also utilized for traditional medicine in East Asian countries, including Korea and China. Our preliminary study demonstrated that ethanolic extract from the Euonymus alatus leaf (EAE) exhibited the strongest antioxidant enzyme-inducing activity among more than 100 kinds of edible tree leaf extracts. This study investigated whether EAE could attenuate the cognitive deficits caused by oxidative stress in mice. Oral intubation of EAE at 100 mg/kg bw or higher resulted in significant improvements to the memory and behavioral impairment induced via i.p. injection of scopolamine. Furthermore, EAE enhanced the expression levels of hippocampal neurotrophic factors such as brain-derived neurotrophic factor (BDNF) and glial cell-derived neurotrophic factor in mice, activated the Nrf2, and the downstream heme oxygenase-1 (HO-1) a quintessential antioxidant enzyme. As rutin (quercetin-3-O-rutinose) was abundantly present in EAE and free quercetin was able to induce defensive antioxidant enzymes in an Nrf2-dependent manner, our findings suggested that quercetin derived from rutin via the intestinal microflora played a significant role in the protection of the mouse hippocampus from scopolamine-induced damage through BDNF-mediated Nrf2 activation, thereby dampening cognitive decline.
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页数:14
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