Separation of conjugated linoleic acid isomers by cyclodextrin-modified micellar electrokinetic chromatography

被引：12

作者：

Liu, XH

Cao, YS ^{[1
]}

Chen, Y

机构：

[1] Nanchang Univ, SinoGerman Joint Res Inst, Nanchang 330047, Peoples R China

[2] Nanchang Univ, Minist Educ, Key Lab Food Sci, Nanchang 330047, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2005年 / 1095卷 / 1-2期

关键词：

micellar electrokinetic chromatography (MEKC); conjugated linoleic acid; isomers; CD-MEKC;

D O I：

10.1016/j.chroma.2005.09.090

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC) method was developed for separating conjugated linoleic acid (CLA) isomers. All the seven CLA isomers (9cis, 11cis-CLA, 9cis, 11trans-CLA, 9trans, 11trans-CLA, 10trans, 12cis-CLA, 11cis, 13cis-CLA, 11cis, 13trans-CLA and 11trans, 13trans-CLA) were completely separated in the optimized conditions (4% (w/v) beta-cyclodextrin (beta-CD), 54 mM sodium dodecyl sulphate (SDS), 80 mM borate (pH 9.0), 8 M urea, 4% (v/v) ethanol, 30 W and 15 degrees C). The CD-MEKC method was superior to the gas chromatographic (GC) and silver-ion high-performance liquid chromatographic (Ag+-HPLC) methods that were generally used in analyzing CLA isomers. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：197 / 200

页数：4

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