Separation of conjugated linoleic acid isomers by cyclodextrin-modified micellar electrokinetic chromatography

被引：12
|
作者：
Liu, XH
Cao, YS [1 ]
Chen, Y
机构：
[1] Nanchang Univ, SinoGerman Joint Res Inst, Nanchang 330047, Peoples R China
[2] Nanchang Univ, Minist Educ, Key Lab Food Sci, Nanchang 330047, Peoples R China
来源：
JOURNAL OF CHROMATOGRAPHY A | 2005年 / 1095卷 / 1-2期
关键词：
micellar electrokinetic chromatography (MEKC); conjugated linoleic acid; isomers; CD-MEKC;
D O I：
10.1016/j.chroma.2005.09.090
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC) method was developed for separating conjugated linoleic acid (CLA) isomers. All the seven CLA isomers (9cis, 11cis-CLA, 9cis, 11trans-CLA, 9trans, 11trans-CLA, 10trans, 12cis-CLA, 11cis, 13cis-CLA, 11cis, 13trans-CLA and 11trans, 13trans-CLA) were completely separated in the optimized conditions (4% (w/v) beta-cyclodextrin (beta-CD), 54 mM sodium dodecyl sulphate (SDS), 80 mM borate (pH 9.0), 8 M urea, 4% (v/v) ethanol, 30 W and 15 degrees C). The CD-MEKC method was superior to the gas chromatographic (GC) and silver-ion high-performance liquid chromatographic (Ag+-HPLC) methods that were generally used in analyzing CLA isomers. (c) 2005 Elsevier B.V. All rights reserved.
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页码：197 / 200
页数：4
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