Modulation of penicillin acylase properties via immobilization techniques:: One-pot chemoenzymatic synthesis of cephamandole from cephalosporin C

被引：95

作者：

Terreni, M

Pagani, G

Ubiali, D

Fernández-Lafuente, R

Mateo, C

Guisán, JM

机构：

[1] Univ Pavia, Pharmaceut Biocatalysis Labs, Dipartimento Chim Farmaceut, I-27100 Pavia, Italy

[2] CSIC, Lab Tecnol Enzimat, Inst Catalisis, E-28049 Madrid, Spain

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2001年 / 11卷 / 18期

关键词：

D O I：

10.1016/S0960-894X(01)00463-2

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The modulation of penicillin G acylase (PGA) properties via immobilization techniques has been performed studying the acylation of 7-aminocephalosporanic acid with R-mandelic acid methyl ester. PGA from Escherichia coli, immobilized onto agarose activated with glycidol (glyoxyl-agarose), has been used for the design of a novel one-pot synthesis of Cephamandole in aqueous medium and without isolation of intermediates, through three consecutive biotransformations catalyzed by D-amino acid oxidase, glutaryl acylase and PGA. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：2429 / 2432

页数：4

共 12 条

[1] IMMOBILIZATION-STABILIZATION OF PENICILLIN-G ACYLASE FROM ESCHERICHIA-COLI [J].