Geographic Distribution of Leishmania Species in Ecuador Based on the Cytochrome B Gene Sequence Analysis

被引：25

作者：

Kato, Hirotomo ^{[1
,2
]}

Gomez, Eduardo A. ^{[3
]}

Martini-Robles, Luiggi ^{[4
]}

Muzzio, Jenny ^{[4
]}

Velez, Lenin ^{[3
]}

Calvopina, Manuel ^{[5
]}

Romero-Alvarez, Daniel ^{[5
]}

Mimori, Tatsuyuki ^{[6
]}

Uezato, Hiroshi ^{[7
]}

Hashiguchi, Yoshihisa ^{[3
]}

机构：

[1] Jichi Med Univ, Dept Infect & Immun, Div Med Zool, Shimotsuke, Tochigi, Japan

[2] Hokkaido Univ, Grad Sch Vet Med, Dept Dis Control, Parasitol Lab, Sapporo, Hokkaido 060, Japan

[3] Univ Catolica Santiago Guayaquil, Fac Ciencias Med, Dept Parasitol & Med Trop, Guayaquil, Ecuador

[4] Insitituto Invest Salud Publ, Dept Parasitol, Guayaquil, Ecuador

[5] Univ Cent Ecuador, Fac Med, Ctr Biomed, Quito, Ecuador

[6] Kumamoto Univ, Grad Sch Hlth Sci, Fac Life Sci, Dept Microbiol, Kumamoto, Japan

[7] Univ Ryukyus, Fac Med, Dept Dermatol, Okinawa, Japan

来源：

PLOS NEGLECTED TROPICAL DISEASES | 2016年 / 10卷 / 07期

关键词：

GIEMSA-STAINED SLIDES; CUTANEOUS LEISHMANIASIS; MOLECULAR DIAGNOSIS; NATURAL INFECTION; VIANNIA NAIFFI; FTA CARD; IDENTIFICATION; AMPLIFICATION; SENSITIVITY; MEXICANA;

D O I：

10.1371/journal.pntd.0004844

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

A countrywide epidemiological study was performed to elucidate the current geographic distribution of causative species of cutaneous leishmaniasis (CL) in Ecuador by using FTA card-spotted samples and smear slides as DNA sources. Putative Leishmania in 165 samples collected from patients with CL in 16 provinces of Ecuador were examined at the species level based on the cytochrome b gene sequence analysis. Of these, 125 samples were successfully identified as Leishmania (Viannia) guyanensis, L. (V.) braziliensis, L. (V.) naiffi, L. (V.) lainsoni, and L. (Leishmania) mexicana. Two dominant species, L. (V.) guyanensis and L. (V.) braziliensis, were widely distributed in Pacific coast subtropical and Amazonian tropical areas, respectively. Recently reported L. (V.) naiffi and L. (V.) lainsoni were identified in Amazonian areas, and L. (L.) mexicana was identified in an Andean highland area. Importantly, the present study demonstrated that cases of L. (V.) braziliensis infection are increasing in Pacific coast areas.

引用

页数：10

共 28 条

[21] DNA extraction and amplification of Leishmania from archived, Giemsa-stained slides, for the diagnosis of cutaneous leishmaniasis by PCR [J].

Motazedian, H ;

Karamian, M ;

Noyes, HA ;

Ardehali, S .

ANNALS OF TROPICAL MEDICINE AND PARASITOLOGY, 2002, 96 (01) :31-34

[22] A rapid molecular diagnosis of cutaneous leishmaniasis by colorimetric malachite green-loop-mediated isothermal amplification (LAMP) combined with an FTA card as a direct sampling tool [J].

Nzelu, Chukwunonso O. ;

Caceres, Abraham G. ;

Guerrero-Quincho, Silvia ;

Tineo-Villafuerte, Edwin ;

Rodriquez-Delfin, Luis ;

Mimori, Tatsuyuki ;

Uezato, Hiroshi ;

Katakura, Ken ;

Gomez, Eduardo A. ;

Guevara, Angel G. ;

Hashiguchi, Yoshihisa ;

Kato, Hirotomo .

ACTA TROPICA, 2016, 153 :116-119

[23] An analysis of reported cases of leishmaniasis in the southern Ecuadorian Amazon region, 1986-2012 [J].

Olalla, Hector R. ;

Velez, Lenin N. ;

Kato, Hirotomo ;

Hashiguchi, Kazue ;

Caceres, Abraham G. ;

Gomez, Eduardo A. ;

Zambrano, Flavio C. ;

Romero-Alvarez, Daniel A. ;

Guevara, Angel G. ;

Hashiguchi, Yoshihisa .

ACTA TROPICA, 2015, 146 :119-126

[24] Molecular diagnosis of leishmaniasis: Current status and future applications [J].

Reithinger, Richard ;

Dujardin, Jean-Claude .

JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (01) :21-25

[25] Is it time to revise the nomenclature of Leishmania? [J].

Schoenian, Gabriele ;

Mauricio, Isabel ;

Cupolillo, Elisa .

TRENDS IN PARASITOLOGY, 2010, 26 (10) :466-469

[26] MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods [J].

Tamura, Koichiro ;

Peterson, Daniel ;

Peterson, Nicholas ;

Stecher, Glen ;

Nei, Masatoshi ;

Kumar, Sudhir .

MOLECULAR BIOLOGY AND EVOLUTION, 2011, 28 (10) :2731-2739

[27] CLUSTAL-W - IMPROVING THE SENSITIVITY OF PROGRESSIVE MULTIPLE SEQUENCE ALIGNMENT THROUGH SEQUENCE WEIGHTING, POSITION-SPECIFIC GAP PENALTIES AND WEIGHT MATRIX CHOICE [J].

THOMPSON, JD ;

HIGGINS, DG ;

GIBSON, TJ .

NUCLEIC ACIDS RESEARCH, 1994, 22 (22) :4673-4680

[28] Diagnosis of cutaneous leishmaniasis [J].

Vega-López, F .

CURRENT OPINION IN INFECTIOUS DISEASES, 2003, 16 (02) :97-101

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