Deletion of a gene encoding an amino acid transporter in the midgut membrane causes resistance to a Bombyx parvo-like virus

被引：74

作者：

Ito, Katsuhiko ^{[1
,2
]}

Kidokoro, Kurako ^{[1
]}

Sezutsu, Hideki ^{[3
]}

Nohata, Junko ^{[1
]}

Yamamoto, Kimiko ^{[1
]}

Kobayashi, Isao ^{[3
]}

Uchino, Keiro ^{[3
]}

Kalyebi, Andrew ^{[1
]}

Eguchi, Ryokitsu ^{[1
]}

Hara, Wajiro ^{[1
]}

Tamura, Toshiki ^{[3
]}

Katsuma, Susumu ^{[2
]}

Shimada, Toru ^{[2
]}

Mita, Kazuei ^{[1
]}

Kadono-Okuda, Keiko ^{[1
]}

机构：

[1] Natl Inst Agrobiol Sci, Div Insect Sci, Tsukuba, Ibaraki 3058634, Japan

[2] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Agr & Environm Biol, Bunkyo Ku, Tokyo 1138657, Japan

[3] Natl Inst Agrobiol Sci, Transgen Silkworm Res Ctr, Tsukuba, Ibaraki 3058634, Japan

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2008年 / 105卷 / 21期

关键词：

virus resistance; positional cloning; transgenesis;

D O I：

10.1073/pnas.0711841105

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Bombyx mori densovirus type 2 (BmDNV-2), a parvo-like virus, replicates only in midgut columnar cells and causes fatal disease. The resistance expressed in some silkworm strains against the virus is determined by a single gone, nsd-2, which is characterized as nonsusceptibility irrespective of the viral dose. However, the responsible gene has been unknown. We isolated the nsd-2 gene by positional cloning. The virus resistance is caused by a 6-kb deletion in the ORF of a gene encoding a 12-pass transmembrane protein, a member of an amino acid transporter family, and expressed only in midgut. Germ-line transformation with a wildtype transgene expressed in the midgut restores susceptibility, showing that the defective membrane protein is responsible for resistance. Cumulatively, our data show that the membrane protein is a functional receptor for BmDNV-2. This is a previously undescribed report of positional cloning of a mutant gene in Bombyx and isolation of an absolute virus resistance gene in insects.

引用

页码：7523 / 7527

页数：5

共 24 条

[1] Identification and genetic mapping of RAPD markers linked to the densonucleosis refractoriness gene, nsd-2, in the silkworm, Bombyx mori [J].

Abe, H ;

Sugasaki, T ;

Kanehara, M ;

Shimada, T ;

Gomi, SJ ;

Ohbayashi, F ;

Oshiki, T .

GENES & GENETIC SYSTEMS, 2000, 75 (02) :93-96

[2] Rapid emergence of baculovirus resistance in codling moth due to dominant, sex-linked inheritance [J].

Asser-Kaiser, S. ;

Fritsch, E. ;

Undorf-Spahn, K. ;

Kienzle, J. ;

Eberle, K. E. ;

Gund, N. A. ;

Reineke, A. ;

Zebitz, C. P. W. ;

Heckel, D. G. ;

Huber, J. ;

Jehle, J. A. .

SCIENCE, 2007, 317 (5846) :1916-1918

[3]

Bergoin M, 2000, CONTRIB MICROBIOL, V4, P12

[4] ERYTHROCYTE-P ANTIGEN - CELLULAR RECEPTOR FOR B19 PARVOVIRUS [J].

BROWN, KE ;

ANDERSON, SM ;

YOUNG, NS .

SCIENCE, 1993, 262 (5130) :114-117

[5] Densoviruses for control and genetic manipulation of mosquitoes [J].

Carlson, Jonathan ;

Suchman, Erica ;

Buchatsky, Leonid .

INSECT VIRUSES: BIOTECHNOLOGICAL APPLICATIONS, 2006, 68 :361-+

[6] Cloning and characterization of a potassium-coupled amino acid transporter [J].

Castagna, M ;

Shayakul, C ;

Trotti, D ;

Sacchi, VF ;

Harvey, WR ;

Hediger, MA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (09) :5395-5400

[7]

Eguchi R., 2007, SANSHI KONTYU BIOTEC, P159

[8] A novel electrogenic amino acid transporter is activated by K+ or Na+, is alkaline pH-dependent, and is Cl--independent [J].

Feldman, DH ;

Harvey, WR ;

Stevens, BR .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (32) :24518-24526

[9]

Furuta Y, 1995, B NATL I SERIC ENTOM, V15, P119

[10]

Furuta Y, 1995, ACTA SERICULT ENTOMO, V15, P119

← 1 2 3 →