Bioaccumulation and toxicity of CdSe/ZnS quantum dots in Phanerochaete chrysosporium

被引:24
作者
Hu, Liang [1 ,2 ]
Zeng, Guangming [1 ,2 ]
Chen, Guiqiu [1 ,2 ]
Huang, Zhenzhen [1 ,2 ]
Wan, Jia [1 ,2 ]
Chen, Anwei [3 ]
Yu, Zhigang [1 ,2 ]
Yang, Jiangli [1 ,2 ]
He, Kai [1 ,2 ]
Qin, Lei [1 ,2 ]
机构
[1] Hunan Univ, Coll Environm Sci & Engn, Changsha 410082, Hunan, Peoples R China
[2] Hunan Univ, Minist Educ, Key Lab Environm Biol & Pollut Control, Changsha 410082, Hunan, Peoples R China
[3] Hunan Agr Univ, Coll Resources & Environm, Changsha 410128, Hunan, Peoples R China
基金
中国国家自然科学基金;
关键词
CdSe/ZnS quantum dots; Toxicity; Reactive oxygen species; Confocal laser scanning microscopy; Phanerochaete chrysosporium; MAGNETIC NANOPARTICLES; PB IMMOBILIZATION; LANDFILL LEACHATE; OXIDATIVE STRESS; PLASMA-MEMBRANE; CELLULAR UPTAKE; WASTE-WATER; ADSORPTION; REMOVAL; CYTOTOXICITY;
D O I
10.1016/j.colsurfb.2017.08.006
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
The growing potential of quantum dots (QDs) in biomedical applications has raised considerable concerns regarding their toxicological impact. Consequently, there has been a need to understand the underlying toxicity mechanism of QDs. In this work, we comprehensively investigated the bioaccumulation and toxicity of three CdSe/ZnS QDs (COOH CdSe/ZnS 525, NH2 CdSe/ZnS 525, and NH2 CdSe/ZnS 625) in Phanerochaete chrysosporium (P. chrysosporium) using confocal laser scanning microscopy, reactive oxygen species (ROS) measurements, and cell viability assays. Confocal laser scanning microscopy analytical results indicated that all the CdSe/ZnS QDs, with the concentration ranging from 10 to 80 nM, could accumulate largely in the hyphae and induce the generation of ROS, showing a direct toxicity to P. chrysosporium. And the bioaccumulation and toxicity of CdSe/ZnS QDs presented dose-dependent and time-dependent effects on P. chrysosporium. Furthermore, the CdSe/ZnS QDs-induced cytotoxicity was also related to their physicochemical properties, including particle size and surface charges: NH2 CdSe/ZnS 525 with small size was more cytotoxic as compared to NH2 CdSe/ZnS 625 with large size, and the smaller negative charged NH2 CdSe/ZnS 525 resulted in greater cytotoxicity than the larger negative charged COOH CdSe/ZnS 525. The obtained results provide valuable information for exploring and understanding of toxicity mechanism of QDs in living cells. (C) 2017 Published by Elsevier B.V.
引用
收藏
页码:303 / 311
页数:9
相关论文
共 50 条
[41]   Analyzed the toxicity of Ganaxolide on Phanerochaete chrysosporium by AFLP [J].
Huang, Wei ;
Liu, Changbing ;
Lin, Yu ;
Xiong, Hongxia ;
Hu, Jianbo .
ENVIRONMENTAL ENGINEERING, PTS 1-4, 2014, 864-867 :266-270
[42]   Influence of heavy-metals toxicity on the growth of Phanerochaete chrysosporium [J].
Falih, AM .
BIORESOURCE TECHNOLOGY, 1997, 60 (01) :87-90
[43]   Toxicity Evaluation of Quantum Dots (ZnS and CdS) Singly and Combined in Zebrafish (Danio rerio) [J].
Matos, Beatriz ;
Martins, Marta ;
Samamed, Antonio Cid ;
Sousa, David ;
Ferreira, Isabel ;
Diniz, Mario S. .
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH, 2020, 17 (01)
[44]   The role of elevated autophagy on the synaptic plasticity impairment caused by CdSe/ZnS quantum dots [J].
Chen, Liang ;
Miao, Yanyan ;
Chen, Lin ;
Jin, Peipei ;
Zha, Yingying ;
Chai, Yuming ;
Zheng, Fang ;
Zhang, Yunjiao ;
Zhou, Wei ;
Zhang, Jigui ;
Wen, Longping ;
Wang, Ming .
BIOMATERIALS, 2013, 34 (38) :10172-10181
[45]   Comparative assessment of the phase transfer behaviour of InP/ZnS and CuInS/ZnS quantum dots and CdSe/ZnS quantum dots under varying environmental conditions [J].
Marsan, Dilem ;
Sengul, Hatice ;
Ozdil, Ayse Muge Andac .
ENVIRONMENTAL SCIENCE-NANO, 2019, 6 (03) :879-891
[46]   Thiol Antioxidant-Functionalized CdSe/ZnS Quantum Dots: Synthesis, Characterization, Cytotoxicity [J].
Zheng, Hong ;
Mortensen, Luke J. ;
DeLouise, Lisa A. .
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, 2013, 9 (03) :382-392
[47]   Dissecting common and divergent molecular pathways elicited by CdSe/ZnS quantum dots in freshwater and marine sentinel invertebrates [J].
Ambrosone, Alfredo ;
Roopin, Modi ;
Pelaz, Beatriz ;
Abdelmonem, Abuelmagd M. ;
Ackermann, Lisa-Maria ;
Mattera, Lucia ;
Allocca, Mariateresa ;
Tino, Angela ;
Klapper, Markus ;
Parak, Wolfgang J. ;
Levy, Oren ;
Tortiglione, Claudia .
NANOTOXICOLOGY, 2017, 11 (02) :289-303
[48]   Highly sensitive electrochemical detection of potential cytotoxicity of CdSe/ZnS quantum dots using neural cell chip [J].
Kim, Tae-Hyung ;
El-Said, Waleed Ahmed ;
Choi, Jeong-Woo .
BIOSENSORS & BIOELECTRONICS, 2012, 32 (01) :266-272
[49]   Metabolic alteration of Tetrahymena thermophila exposed to CdSe/ZnS quantum dots to respond to oxidative stress and lipid damage [J].
He, Jie ;
Wang, Zhi-zheng ;
Li, Chen-hong ;
Xu, Hai-long ;
Pan, Hong-zhi ;
Zhao, Yu-xia .
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2023, 1867 (01)
[50]   Toxicological effects of CdSe/ZnS quantum dots on marine planktonic organisms [J].
Zhou, Chao ;
Vitiello, Valentina ;
Pellegrini, David ;
Wu, Changwen ;
Morelli, Elisabetta ;
Buttino, Isabella .
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2016, 123 :26-31