Molecular Screening and Characterization of Shiga Toxin-producing Escherichia coli By Multiplex PCR Assays for stx1, stx2, eaeA, H7 in Raw Milk

被引：1
作者：
Koev, Koycho [1 ]
Zhelev, Georgi [1 ]
Marutsov, Plamen [1 ]
Gospodinova, Krasimira [1 ]
Petrov, Vladimir [1 ]
Stoyanchev, Todor [2 ]
机构：
[1] Trakia Univ, Dept Vet Microbiol Infect & Parsit Dis, Stara Zagora, Bulgaria
[2] Trakia Univ, Dept Food Hyg Technol & Control, Vet Legislat & Management, Stara Zagora, Bulgaria
来源：
KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI | 2019年 / 25卷 / 02期
关键词：
Escherichia coli O157:H7; Dairy farms; Bulk milk; Enrichment; PCR; DAIRY; IDENTIFICATION; O157/H7; O157-H7; GROWTH; BOVINE; WATER;
D O I：
10.9775/kvfd.2018.20373
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
The presence of Shiga-toxin producing Escherichia coli (STEC) was evaluated in bulk milk from dairy cattle farms in Bulgaria. From 20 regional farms only 4 were selected as a source for milk sampling on the basis of their annual STEC shedding in feces. All the samples were PCR analyzed after selective enrichment. Positive samples were detected in subsequent molecular testing as: two of samples (9.08%) were positive for stx(1), one sample (4.54%) for stx(2), 10 samples (45.54%) for eae and 12 (54.54%) for H7 coding genes.
引用
收藏
页码：271 / 275
页数：5
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