Anisotropic Rod-Shaped Particles Influence Injectable Granular Hydrogel Properties and Cell Invasion

被引：128

作者：

Qazi, Taimoor H. ^{[1
]}

Wu, Jingyu ^{[2
]}

Muir, Victoria G. ^{[1
]}

Weintraub, Shoshana ^{[1
]}

Gullbrand, Sarah E. ^{[3
,4
]}

Lee, Daeyeon ^{[2
]}

Issadore, David ^{[1
,5
]}

Burdick, Jason A. ^{[1
]}

机构：

[1] Univ Penn, Sch Engn & Appl Sci, Dept Bioengn, Philadelphia, PA 19104 USA

[2] Univ Penn, Dept Chem & Biomol Engn, Philadelphia, PA 19104 USA

[3] Corporal Michael J Crescenz VA Med Ctr, Translat Musculoskeletal Res Ctr, Philadelphia, PA 19104 USA

[4] Univ Penn, McKay Orthopaed Res Lab, Dept Orthopaed Surg, Philadelphia, PA 19104 USA

[5] Univ Penn, Dept Elect & Syst Engn, Philadelphia, PA 19104 USA

来源：

ADVANCED MATERIALS | 2022年 / 34卷 / 12期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

angiogenesis; biomaterials; elongated particles; endogenous repair; jamming; microgels; MICROGELS;

D O I：

10.1002/adma.202109194

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Granular hydrogels have emerged as a new class of injectable and porous biomaterials that improve integration with host tissue when compared to solid hydrogels. Granular hydrogels are typically prepared using spherical particles and this study considers whether particle shape (i.e., isotropic spheres vs anisotropic rods) influences granular hydrogel properties and cellular invasion. Simulations predict that anisotropic rods influence pore shape and interconnectivity, as well as bead transport through granular assemblies. Photo-cross-linkable norbornene-modified hyaluronic acid is used to produce spherical and rod-shaped particles using microfluidic droplet generators and formed into shear-thinning and self-healing granular hydrogels, with particle shape influencing mechanics and injectability. Rod-shaped particles form granular hydrogels that have anisotropic and interconnected pores, with pore size and number influenced by particle shape and degree of packing. Robust in vitro sprouting of endothelial cells from embedded cellular spheroids is observed with rod-shaped particles, including higher sprouting densities and sprout lengths when compared to hydrogels with spherical particles. Cell and vessel invasion into granular hydrogels when injected subcutaneously in vivo are significantly greater with rod-shaped particles, whereas a gradient of cellularity is observed with spherical particles. Overall, this work demonstrates potentially superior functional properties of granular hydrogels with rod-shaped particles for tissue repair.

引用

页数：12

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