Influence of Implant Material and Surface on Differentiation and Proliferation of Human Adipose-Derived Stromal Cells

被引:13
|
作者
Jung, Susanne [1 ]
Bohner, Lauren [1 ]
Hanisch, Marcel [1 ]
Kleinheinz, Johannes [1 ]
Sielker, Sonja [1 ]
机构
[1] Univ Hosp Muenster, Res Unit Vasc Biol Oral Struct VABOS, Dept Craniomaxillofacial Surg, D-48149 Munster, Germany
关键词
hADSC; tissue regeneration; implants; titanium; zirconia; MESENCHYMAL STEM-CELLS; OSTEOGENIC DIFFERENTIATION; ZIRCONIA; BONE;
D O I
10.3390/ijms19124033
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
For the guided regeneration of periimplant hard and soft tissues, human adipose-derived stromal cells (hADSC) seem to be a promising source for mesenchymal stromal cells. For this, the proliferation and differentiation of hADSC were evaluated on titanium and zirconia dental implants with different surface treatments. Results were compared to edaphic cells as human osteoblasts (hOB) and human gingival fibroblasts (HGF). Primary cells were cultured on (1) titanium implants with a polished surface (Ti-PT), (2) sandblasted and acid-etched titanium (Ti-SLA), (3) sandblasted and alkaline etched zirconia (ZrO2-ZLA) and (4) machined zirconia (ZrO2-M). The cell proliferation and differentiation on osteogenic lineage were assessed after 1, 7 and 14 days. Statistical analysis was performed by one-way ANOVA and a modified Levene test with a statistical significance at p = 0.05. PostHoc tests were performed by Bonferroni-Holm. Zirconia dental implants with rough surface (ZrO2-ZLA) showed the highest proliferation rates (p = 0.048). The osteogenic differentiation occurred early for zirconia and later for titanium implants, and it was enhanced for rough surfaces in comparison to polished/machined surfaces. Zirconia was more effective to promote the proliferation and differentiation of hADSCs in comparison to titanium. Rough surfaces were able to improve the biological response for both zirconia and titanium.
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页数:9
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