Enhancing vitamin B12 content in soy-yogurt by Lactobacillus reuteri

被引:65
作者
Gu, Qing [1 ]
Zhang, Chen [1 ]
Song, Dafeng [1 ]
Li, Ping [1 ]
Zhu, Xuan [1 ]
机构
[1] Zhejiang Gongshang Univ, Key Lab Food Microbial Technol Zhejiang Prov, Hangzhou 310018, Zhejiang, Peoples R China
关键词
Lactobacillus reuteri; Vitamin B-12; Glycerol; Fructose; cobT; BACTERIA;
D O I
10.1016/j.ijfoodmicro.2015.04.033
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
More attention from the aged and vegetarians has been paid to soy-product due to its taste, easy digestibility, as well as the association with health. However, soy-product has a defect of low vitamin content, mainly the water-soluble vitamin B-12. This study was to investigate co-fermentation of glycerol and fructose in soy-yogurt to enhance vitamin B-12 production by Lactobacillus reuteri. After a serial combination experiments, the co-fermentation was confirmed to enhance the production of vitamin B-12 up to 18 mu g/100 mL Both supplementations induced the expression of cobT and cbiA and functioned to balance the redox reaction. Meanwhile, high content of fructose supplementation reduced the production of vitamin B-12 and suppressed expression of cobT in bacteria. It was proved that the vitamin B-12 content of this soy-yogurt is higher than other fermented soybean based food and thus can be served as an alternative food for the aged and vegetarians. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码:56 / 59
页数:4
相关论文
共 25 条
[1]   Bioavailability of Vitamin B12 [J].
Allen, Lindsay Helen .
INTERNATIONAL JOURNAL FOR VITAMIN AND NUTRITION RESEARCH, 2010, 80 (4-5) :330-335
[2]   Influence of environmental parameters on production of the acrolein precursor 3-hydroxypropionaldehyde by Lactobacillus reuteri DSMZ 20016 and its accumulation by wine lactobacilli [J].
Bauer, Rolene ;
du Toit, Maret ;
Kossmann, Jens .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2010, 137 (01) :28-31
[3]  
Casas Ivan A., 2000, Microbial Ecology in Health and Disease, V12, P247
[4]   Regiospecific Formation of Cobamide Isomers Is Directed by CobT [J].
Crofts, Terence S. ;
Hazra, Amrita B. ;
Tran, Jennifer L. A. ;
Sokoloyskaya, Olga M. ;
Osadchiy, Vadim ;
Ad, Omer ;
Pelton, Jeffrey ;
Bauer, Stefan ;
Taga, Michiko E. .
BIOCHEMISTRY, 2014, 53 (49) :7805-7815
[5]   FORMATION OF B-VITAMINS BY BACTERIA DURING THE SOAKING PROCESS OF SOYBEANS FOR TEMPE FERMENTATION [J].
DENTER, J ;
BISPING, B .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1994, 22 (01) :23-31
[6]   DETERMINATION OF THE WATER-SOLUBLE VITAMINS B1, B2, B6 AND B12 IN MILK BY HPLC [J].
GAUCH, R ;
LEUENBERGER, U ;
MULLER, U .
ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG, 1992, 195 (04) :312-315
[7]   Concurrent high production of natural folate and vitamin B12 using a co-culture process with Lactobacillus plantarum SM39 and Propionibacterium freudenreichii DF13 [J].
Hugenschmidt, Selina ;
Schwenninger, Susanne Miescher ;
Lacroix, Christophe .
PROCESS BIOCHEMISTRY, 2011, 46 (05) :1063-1070
[8]   VITAMIN-B-12, PRODUCTION BY CITROBACTER-FREUNDII OR KLEBSIELLA-PNEUMONIAE DURING TEMPEH FERMENTATION AND PROOF OF ENTEROTOXIN ABSENCE BY PCR [J].
KEUTH, S ;
BISPING, B .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (05) :1495-1499
[9]   FORMATION OF VITAMINS BY PURE CULTURES OF TEMPE MOLDS AND BACTERIA DURING THE TEMPE SOLID SUBSTRATE FERMENTATION [J].
KEUTH, S ;
BISPING, B .
JOURNAL OF APPLIED BACTERIOLOGY, 1993, 75 (05) :427-434
[10]  
Liu K.S., 2009, SOYBEAN FUNCTIONAL F