Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray

被引：92

作者：

Carter, MG

Sharov, AA

VanBuren, V

Dudekula, DB

Carmack, CE

Nelson, C

Ko, MSH

机构：

[1] NIA, Dev Genom & Aging Sect, Genet Lab, Baltimore, MD 21224 USA

[2] Agilent Technol, Palo Alto, CA 94304 USA

来源：

GENOME BIOLOGY | 2005年 / 6卷 / 07期

关键词：

D O I：

10.1186/gb-2005-6-7-r61

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The ability to quantitatively measure the expression of all genes in a given tissue or cell with a single assay is an exciting promise of gene-expression profiling technology. An in situ-synthesized 60-mer oligonucleotide microarray designed to detect transcripts from all mouse genes was validated, as well as a set of exogenous RNA controls derived from the yeast genome ( made freely available without restriction), which allow quantitative estimation of absolute endogenous transcript abundance.

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页数：12

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共 28 条

[1] Gene Ontology: tool for the unification of biology [J].

Ashburner, M ;

Ball, CA ;

Blake, JA ;

Botstein, D ;

Butler, H ;

Cherry, JM ;

Davis, AP ;

Dolinski, K ;

Dwight, SS ;

Eppig, JT ;

Harris, MA ;

Hill, DP ;

Issel-Tarver, L ;

Kasarskis, A ;

Lewis, S ;

Matese, JC ;

Richardson, JE ;

Ringwald, M ;

Rubin, GM ;

Sherlock, G .

NATURE GENETICS, 2000, 25 (01) :25-29

[2] ANALYSIS OF COMPLEXITY AND DIVERSITY OF MESSENGER-RNA FROM CHICKEN LIVER AND OVIDUCT [J].

AXEL, R ;

FEIGELSON, P ;

SCHUTZ, G .

CELL, 1976, 7 (02) :247-254

[3] 3 ABUNDANCE CLASSES IN HELA-CELL MESSENGER-RNA [J].

BISHOP, JO ;

MORTON, JG ;

ROSBASH, M ;

RICHARDSON, M .

NATURE, 1974, 250 (5463) :199-204

[4] Expression profiling of the schizont and trophozoite stages of Plasmodium falciparum with a long-oligonucleotide microarray -: art. no. R9 [J].

Bozdech, Z ;

Zhu, JC ;

Joachimiak, MP ;

Cohen, FE ;

Pulliam, B ;

DeRisi, JL .

GENOME BIOLOGY, 2003, 4 (02)

[5] ArrayExpress - a public repository for microarray gene expression data at the EBI [J].

Brazma, A ;

Parkinson, H ;

Sarkans, U ;

Shojatalab, M ;

Vilo, J ;

Abeygunawardena, N ;

Holloway, E ;

Kapushesky, M ;

Kemmeren, P ;

Lara, GG ;

Oezcimen, A ;

Rocca-Serra, P ;

Sansone, SA .

NUCLEIC ACIDS RESEARCH, 2003, 31 (01) :68-71

[6] The NIA cDNA Project in mouse stem cells and early embryos [J].

Carter, MG ;

Piao, YL ;

Dudekula, DB ;

Qian, Y ;

VanBuren, V ;

Sharov, AA ;

Tanaka, TS ;

Martin, PR ;

Bassey, UC ;

Stagg, CA ;

Aiba, K ;

Hamatani, T ;

Matoba, R ;

Kargul, GJ ;

Ko, MSH .

COMPTES RENDUS BIOLOGIES, 2003, 326 (10-11) :931-940

[7] In situ-synthesized novel microarray optimized for mouse stem cell and early developmental expression profiling [J].

Carter, MG ;

Hamatani, T ;

Sharov, AA ;

Carmack, CE ;

Qian, Y ;

Aiba, K ;

Ko, NT ;

Dudekula, DB ;

Brzoska, PM ;

Hwang, SS ;

Ko, MSH .

GENOME RESEARCH, 2003, 13 (05) :1011-1021

[8] Measuring absolute expression with microarrays with a calibrated reference sample and an extended signal intensity range [J].

Dudley, AM ;

Aach, J ;

Steffen, MA ;

Church, GM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (11) :7554-7559

[9] Gene Expression Omnibus: NCBI gene expression and hybridization array data repository [J].

Edgar, R ;

Domrachev, M ;

Lash, AE .

NUCLEIC ACIDS RESEARCH, 2002, 30 (01) :207-210

[10] ESTGenes: Alternative splicing from ESTs in Ensembl [J].

Eyras, E ;

Caccamo, M ;

Curwen, V ;

Clamp, M .

GENOME RESEARCH, 2004, 14 (05) :976-987