Regulation of gamma-glutamylcysteine synthetase activity by nerve growth factor

被引:0
|
作者
Pan, ZH [1 ]
PerezPolo, R [1 ]
机构
[1] UNIV TEXAS,MED BRANCH,GALVESTON,TX 77555
关键词
glutathione; nerve growth factor; PC12; gamma-glutamylcysteine synthetase;
D O I
暂无
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Nerve growth factor is a neurotrophic factor that regulates neuronal cell development, maintenance, and injury responses in the peripheral and central nervous system. Nerve growth factor reduces injury owing to oxidative stress in rat pheochromocytoma (PC12) cells by increasing intracellular glutathione, in part owing to its stimulation of the activity of gamma-glutamylcysteine synthetase, which is the rate-limiting enzyme in the synthesis of glutathione. Here we show that nerve growth factor did not increase the activity of gamma-glutamylcysteine synthetase in PC12 cells at the transcriptional level. Rather, nerve growth factor enhanced the stability of gamma-glutamylcysteine synthetase mRNA in PC12 cells. These results suggest that, during oxidative stress, nerve growth factor extended the half-life of gamma-glutamylcysteine synthetase mRNA thus increasing gamma-glutamylcysteine synthetase mRNA levels compared to nerve growth factor-deprived PC12 cells. Copyright (C) 1996 ISDN.
引用
收藏
页码:559 / 566
页数:8
相关论文
共 50 条
  • [31] Regulation of cysteine dioxygenase and γ-glutamylcysteine synthetase is associated with hepatic cysteine level
    Lee, JI
    Londono, M
    Hirschberger, LL
    Stipanuk, MH
    JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 2004, 15 (02) : 112 - 122
  • [32] Overexpression of the regulatory subunit of γ-glutamylcysteine synthetase in HeLa cells increases γ-glutamylcysteine synthetase activity and confers drug resistance
    Tipnis, SR
    Blake, DG
    Shepherd, AG
    McLellan, LI
    BIOCHEMICAL JOURNAL, 1999, 337 : 559 - 566
  • [33] Regulation of γ-glutamylcysteine synthetase expression in response to oxidative stress
    Kondo, T
    Higashiyama, Y
    Goto, S
    Iida, T
    Cho, S
    Iwanaga, M
    Mori, K
    Tani, M
    Urata, Y
    FREE RADICAL RESEARCH, 1999, 31 (04) : 325 - 334
  • [34] Altered expression of gamma-glutamylcysteine synthetase, metallothionein and topoisomerase I or II during acquisition of drug resistance to cisplatin in human ovarian cancer cells
    Kikuchi, Y
    Hirata, J
    Yamamoto, K
    Ishii, K
    Kita, T
    Kudoh, K
    Tode, T
    Nagata, I
    Taniguchi, K
    Kuwano, M
    JAPANESE JOURNAL OF CANCER RESEARCH, 1997, 88 (02): : 213 - 217
  • [35] Evaluating the Potential of Gamma-Glutamylcysteine and Glutathione as Substitutes for SO2 in White Wine
    Guruk, Mumine
    Fickers, Patrick
    Agirman, Bilal
    Darici, Merve
    Erten, Huseyin
    FOOD SCIENCE & NUTRITION, 2025, 13 (02):
  • [36] The gene for gamma-glutamylcysteine synthetase from Thiobacillus ferrooxidans has low homology to its Escherichia coli equivalent and is linked to the gene for citrate synthase
    Powles, R
    Deane, S
    Rawlings, D
    MICROBIOLOGY-UK, 1996, 142 : 2543 - 2548
  • [37] ANTICATARACTOGENIC PROPERTY OF GAMMA-GLUTAMYLCYSTEINE ETHYL-ESTER IN AN ANIMAL-MODEL OF CATARACT
    OHTSU, A
    KITAHARA, S
    FUJII, K
    OPHTHALMIC RESEARCH, 1991, 23 (01) : 51 - 58
  • [38] Co-amplification of the gamma-glutamylcysteine synthetase gene gsh1 and of the ABC transporter gene pgpA in arsenite-resistant Leishmania tarentolae
    Grondin, K
    Haimeur, A
    Mukhopadhyay, R
    Rosen, BP
    Ouellette, M
    EMBO JOURNAL, 1997, 16 (11) : 3057 - 3065
  • [39] THE CARDIOPROTECTIVE EFFECT OF GAMMA-GLUTAMYLCYSTEINE ETHYL-ESTER DURING CORONARY REPERFUSION IN CANINE HEARTS
    NISHINAKA, Y
    KITAHARA, S
    SUGIYAMA, S
    YOKOTA, M
    SAITO, H
    OZAWA, T
    BRITISH JOURNAL OF PHARMACOLOGY, 1991, 104 (04) : 805 - 810
  • [40] In vivo amelioration of adriamycin induced oxidative stress in plasma by gamma-glutamylcysteine ethyl ester (GCEE)
    Aluise, Christopher D.
    Clair, Daret St.
    Vore, Mary
    Butterfield, D. Allan
    CANCER LETTERS, 2009, 282 (01) : 25 - 29