Microdialysis of galanin in rat spinal cord: in vitro and in vivo studies

被引：0

作者：

Blakeman, KH ^{[1
]}

Wiesenfeld-Hallin, Z ^{[1
]}

Alster, P ^{[1
]}

机构：

[1] Huddinge Univ Hosp, Karolinska Inst, Dept Med Lab Sci & Technol, Div Clin Neurophysiol, S-14186 Huddinge, Sweden

来源：

EXPERIMENTAL BRAIN RESEARCH | 2001年 / 139卷 / 03期

关键词：

neuropeptides; radioimmunoassay; microdialysis; galanin; spinal cord;

D O I：

暂无

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The release of the neuropeptide galanin was measured with microdialysis in the dorsal horn of the rat spinal cord. Recovery of unlabeled galanin-like immunoreactivity (GAL-LI) in vitro was measured with radioimmunoassay (RIA) and was found to be 1.4-1.5%. In vivo galanin level was close to the detection level of the RIA under basal conditions. The peptide was released in the dorsal horn during electrical stimulation of the sciatic nerve. The nerve was stimulated for 30 min at a stimulus strength that activated A- and C-fibers. Thus, release of galanin in the spinal cord of the rat is possible to measure with the microdialysis technique.

引用

页码：354 / 358

页数：5

共 25 条

[1] METABOLISM OF GALANIN AND GALANIN(1-16) IN ISOLATED CEREBROSPINAL-FLUID AND SPINAL-CORD MEMBRANES FROM RAT [J].