Biochemical sniffer with choline oxidase for measurement of choline vapour

被引：11

作者：

Kudo, Hiroyuki ^{[1
]}

Goto, Teruyoshi ^{[2
]}

Saito, Takao ^{[1
]}

Saito, Hirokazu ^{[1
]}

Otsuka, Kimio ^{[1
]}

Mitsubayashi, Kohji ^{[1
]}

机构：

[1] Tokyo Med & Dent Univ, Inst Biomat & Bioengn, Dept Biomed Devices & Instrumentat, Chiyoda Ku, Tokyo 1010062, Japan

[2] Tokai Univ, Grad Sch Engn, Kanagawa 2591100, Japan

来源：

MICROCHIMICA ACTA | 2008年 / 160卷 / 04期

关键词：

biosensor; enzyme immobilization; choline; amperometoric; gas sensor;

D O I：

10.1007/s00604-007-0796-x

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An enzyme-based gas sensor (bio-sniffer) for choline vapour was fabricated and tested. The bio-sniffer was constructed using a Clark-type dissolved oxygen electrode and an enzyme (choline oxidase) immobilized membrane. This bioelectronic device measures choline concentration by the oxygen consumption induced by an enzyme reaction of choline oxidase. As the assessment of sensor performances, the calibration curves for choline in the liquid and gas phases were investigated, respectively. The responses of the bioelectronic device to choline solutions of various concentrations were related within a range from 5.00 to 700 mu mol center dot L-1 with a correlation coefficient of 0.999. On the other hand, the bio-sniffer for choline vapour was placed into a gas-measuring chamber and calibrated using gas detection tubes. The calibration range was 1.00-30.0 ppm (correlation coefficient: 0.996). The response time for choline vapour was approximately 15% slower than that of biosensor for choline solution. These results indicate that the bio-sniffer is useful to monitor colourless and odourless choline gas released from coating compositions including choline.

引用

页码：421 / 426

页数：6

共 29 条

[1] Amperometric enzyme electrodes .8. Enzyme electrodes for choline plus betaine aldehyde and hypoxanthine plus xanthine. A kinetic model for a one-enzyme sequential substrate system [J].

Albery, WJ ;

Driscoll, BJ ;

Kalia, YN .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1995, 399 (1-2) :13-20

[2] A wireless, remote query glucose biosensor based on a pH-sensitive polymer [J].

Cai, QY ;

Zeng, KF ;

Ruan, CM ;

Desai, TA ;

Grimes, CA .

ANALYTICAL CHEMISTRY, 2004, 76 (14) :4038-4043

[3] Characterization of a planar L-glutamate amperometric biosensor immobilized with a photo-crosslinkable polymer membrane [J].

Chang, Ku-Shang ;

Chang, Chen-Kai ;

Chou, Su-Fen ;

Han, Hsei-Chen ;

Chen, Chien-Yuan .

SENSORS AND ACTUATORS B-CHEMICAL, 2007, 122 (01) :195-203

[4] DETERMINATION OF ENDOGENOUS ACETYLCHOLINE-RELEASE IN FREELY MOVING RATS BY TRANSSTRIATAL DIALYSIS COUPLED TO A RADIOENZYMATIC ASSAY - EFFECT OF DRUGS [J].

CONSOLO, S ;

WU, CF ;

FIORENTINI, F ;

LADINSKY, H ;

VEZZANI, A .

JOURNAL OF NEUROCHEMISTRY, 1987, 48 (05) :1459-1465

[5]

CUADRA G, 1994, J PHARMACOL EXP THER, V270, P277

[6] Potentiometric biosensors based on ISFETs and immobilized cholinesterases [J].

Dzyadevych, SV ;

Arkhypova, VN ;

Martelet, C ;

Jaffrezic-Renault, N ;

Chovelon, JM ;

El'skaya, AV ;

Soldatkin, AP .

ELECTROANALYSIS, 2004, 16 (22) :1873-1882

[7]

FARIDNIA MH, 1993, ANAL CHIM ACTA, V278, P235

[8] Amperometric microsensors for monitoring choline in the extracellular fluid of brain [J].

Garguilo, MG ;

Michael, AC .

JOURNAL OF NEUROSCIENCE METHODS, 1996, 70 (01) :73-82

[9] Electrosynthesized poly(pyrrole)/poly(2-naphthol) bilayer membrane as an effective anti-interference layer for simultaneous determination of acethylcholine and choline by a dual electrode amperometric biosensor [J].

Guerrieri, A ;

Lattanzio, V ;

Palmisano, F ;

Zambonin, PG .

BIOSENSORS & BIOELECTRONICS, 2006, 21 (09) :1710-1718

[10] DEVELOPMENT OF CHOLINE AND ACETYLCHOLINE PT MICROELECTRODES [J].

HUANG, ZX ;

VILLARTASNOW, R ;

LUBRANO, GJ ;

GUILBAULT, GG .

ANALYTICAL BIOCHEMISTRY, 1993, 215 (01) :31-37

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