HPLC-UV DETERMINATION OF MORPHINE IN HUMAN PLASMA AND ITS APPLICATION TO THE CLINICAL STUDY

被引：0
|
作者：
Szkutnik-Fiedler, Danuta [1 ]
Grzeskowiak, Edmund [1 ]
Gaca, Michal [2 ,3 ]
Borowicz, Magdalena [1 ]
机构：
[1] Poznan Univ Med Sci, Dept Clin Pharm & Biopharm, PL-61861 Poznan, Poland
[2] Poznan Univ Med Sci, Clin Obstet, PL-60535 Poznan, Poland
[3] Poznan Univ Med Sci, Clin Gynecol Anaesthesiol, PL-60535 Poznan, Poland
来源：
ACTA POLONIAE PHARMACEUTICA | 2011年 / 68卷 / 04期
关键词：
high performance liquid chromatography; UV detector; plasma samples; morphine; hysterectomy; pain; TANDEM MASS-SPECTROMETRY; PERFORMANCE LIQUID-CHROMATOGRAPHY; FLUORESCENCE DETECTION; GC-MS; LC-MS; BLOOD; URINE; 6-ACETYLMORPHINE; METABOLITES; CODEINE;
D O I：
暂无
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
A sensitive and specific high-performance liquid chromatography method with ultraviolet detection (HPLC-UV) has been developed for the quantification of morphine sulfate [(5 alpha,6 alpha)-7,8-didehydro-4,5-epoxy-17-methylmorphinan-3,6-dioll, (CAS: 52-26-6) in human plasma. The analyte was extracted from plasma samples with chloroform isopropyl alcohol (90:10, v/v) and analyzed on a Bondapak C-18 column. The calibration curves were linear within the range of 10-150 ng/mL. The lower limit of quantitation was 10 ng/mL with 0.5 mL plasma sample. The mean recovery of the drug from plasma samples was 83.39%. The results from analysis of quality-control samples at concentrations of 30, 75, and 150 ng/mL were indicative of good accuracy and precision. This method was successfully used to analyze morphine in plasma samples of patients after abdominal hysterectomy.
引用
收藏
页码：473 / 479
页数：7
相关论文
共 50 条
  • [21] HPLC-UV ASSAY OF IMATINIB IN HUMAN PLASMA OPTIMIZED FOR BIOEQUIVALENCE STUDIES
    Kaza, Michal
    Piorkowska, Edyta
    Filist, Monika
    Rudzki, Piotr J.
    ACTA POLONIAE PHARMACEUTICA, 2016, 73 (06): : 1495 - 1503
  • [22] A sensitive and validated HPLC-UV method for the quantitative determination of the new antifungal drug isavuconazole in human plasma
    Nannetti, Giulio
    Pagni, Silvana
    Palu, Giorgio
    Loregian, Arianna
    BIOMEDICAL CHROMATOGRAPHY, 2018, 32 (11)
  • [23] Sensitive and rapid HPLC-UV method with back-extraction step for the determination of sildenafil in human plasma
    Al-Hroub, Hamza
    Alkhawaja, Bayan
    Alkhawaja, Eman
    Arafat, Tawfiq
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2016, 1009 : 1 - 6
  • [24] Development and validation of an HPLC-UV detection assay for the determination of rufinamide in human plasma and saliva
    Mazzucchelli, Iolanda
    Rapetti, Manuela
    Fattore, Cinzia
    Franco, Valentina
    Gatti, Giuliana
    Perucca, Emilio
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2011, 401 (03) : 1013 - 1021
  • [25] Optimization and Validation of An Isocratic HPLC-UV Method for the Simultaneous Determination of Five Drugs Used in Combined Cardiovascular Therapy in Human Plasma
    Soltani, Somaieh
    Jouyban, Abolghasem
    ASIAN JOURNAL OF CHEMISTRY, 2011, 23 (04) : 1728 - 1734
  • [26] Therapeutic Drug Monitoring in Oncohematological Patients: A Fast and Accurate HPLC-UV Method for the Quantification of Nilotinib in Human Plasma and Its Clinical Application
    Escudero-Ortiz, Vanesa
    Rodriguez-Lucena, Francisco Jose
    Estan-Cerezo, Gabriel
    Mancheno-Macia, Esther
    Conesa-Garcia, Venancio
    Garcia-Monsalve, Ana
    Soriano-Irigaray, Leticia
    Navarro-Ruiz, Andres
    BIOMEDICINES, 2023, 11 (03)
  • [27] A Simple HPLC-UV Method for the Quantification of Theophylline in Rabbit Plasma and its Pharmacokinetic Application
    Al-Jenoobi, Fahad I.
    Ahad, Abdul
    Mahrous, Gamal M.
    Raish, Mohammad
    Alam, Mohd Aftab
    Al-Mohizea, Abdullah M.
    JOURNAL OF CHROMATOGRAPHIC SCIENCE, 2015, 53 (10) : 1765 - 1770
  • [28] Simultaneous analysis of diazepam and its metabolites in rat plasma and brain tissue by HPLC-UV and SPE
    Mercolini, Laura
    Mandrioli, Roberto
    Iannello, Carmelina
    Matrisciano, Francesco
    Nicoletti, Ferdinando
    Raggi, Maria Augusta
    TALANTA, 2009, 80 (01) : 279 - 285
  • [29] Determination of Retinol, α-Tocopherol, Lycopene, and β-Carotene in Human Plasma Using HPLC with UV-Vis Detection: Application to a Clinical Study
    Kand'ar, Roman
    Novotna, Pavla
    Drabkova, Petra
    JOURNAL OF CHEMISTRY, 2013, 2013
  • [30] OPTIMIZATION AND VALIDATION OF HPLC-UV METHOD FOR SIMULTANEOUS DETERMINATION OF OMEPRAZOLE AND ITS METABOLITES IN HUMAN PLASMA: EFFECTS OF VARIOUS EXPERIMENTAL CONDITIONS AND PARAMETERS
    Ahmad, Lateef
    Iqbal, Zafar
    Nazir, Shabnam
    Shah, Yasar
    Khan, Abad
    Khan, Muhammad I.
    Nasir, Fazli
    Khan, Abbas
    JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2011, 34 (15) : 1488 - 1501
12345