The CRISPR-cas system promotes antimicrobial resistance in Campylobacter jejuni

被引：36
|
作者：
Shabbir, Muhammad A. B. [1 ]
Wu, Qin [2 ,3 ]
Shabbir, Muhammad Z. [4 ]
Sajid, Abdul [1 ,6 ]
Ahmed, Saeed [1 ]
Sattar, Adeel [5 ]
Tang, Yanping [1 ]
Li, Jun [1 ]
Maan, Muhammad K. [1 ]
Hao, Haihong [1 ,2 ,3 ]
Yuan, Zonghui [1 ,2 ,3 ]
机构：
[1] Huazhong Agr Univ, China MOA Lab Risk Assessment Qual & Safety Lives, Wuhan 430070, Hubei, Peoples R China
[2] Huazhong Agr Univ, Natl Reference Lab Vet Drug Residues, Wuhan 430070, Hubei, Peoples R China
[3] Huazhong Agr Univ, MOA Key Lab Detect Vet Drug Residues Foods, Wuhan 430070, Hubei, Peoples R China
[4] Univ Vet & Anim Sci, Qual Operat Lab, Lahore 54600, Pakistan
[5] Univ Vet & Anim Sci, Dept Pharmacol & Toxicol, Lahore 54600, Pakistan
[6] Abdul Wali Khan Univ, Coll Vet Sci & Anim Husb, Mardan 23200, Pakistan
来源：
FUTURE MICROBIOLOGY | 2018年 / 13卷 / 16期
基金：
中国国家自然科学基金;
关键词：
Campylobacter jejuni; cas9; gene; CRISPR-cas system; differentially expressed genes; transcriptome analysis; HORIZONTAL GENE-TRANSFER; OXIDATIVE STRESS; MACROLIDE RESISTANCE; FLUOROQUINOLONES; INTERFERENCE; ERYTHROMYCIN; INDUCTION; DEFENSE; COLI; RNA;
D O I：
10.2217/fmb-2018-0234
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Aim: The purpose of current study is to find out relationship between cas9 gene and antimicrobial resistance in Campylobacter jejuni NCTC11168. Materials & methods: The involvement of the cas9 gene in antimicrobial resistance of C. jejuni was determined by assessment of minimum inhibitory concentration, clustered regularly interspaced short palindromic repeats (CRISPR)-cas gene expression in standard strains, in vitro resistance development and transcriptome analysis of a cas9 deletion mutant and wild strains. Results: Increased expression of CRISPR-related genes was observed in standard strains. We also observed that cas9 mutant strain is more sensitive to antibiotics than its wild strain. Transcriptome analysis revealed that cas9 gene regulate several genes to promote antimicrobial resistance in C. jejuni. Conclusion: CRISPR-cas system plays role in the enhancement of antimicrobial resistance in C. jejuni.
引用
收藏
页码：1757 / 1774
页数：18
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