Calcium signaling involved in bovine herpesvirus 1 replication in MDBK cells

被引：3
作者：
Zhu, L. [1 ,2 ]
Huang, L. [1 ,2 ]
Zhu, Y. [1 ,2 ]
Ding, X. [1 ,2 ,3 ]
Zhu, G. [1 ,2 ]
机构：
[1] Yangzhou Univ, Coll Vet Med, 48 Wenhui East Rd, Yangzhou 225009, Jiangsu, Peoples R China
[2] Jiangsu Coinnovat Ctr Prevent & Control Important, 48 Wenhui East Rd, Yangzhou 225009, Jiangsu, Peoples R China
[3] Yangzhou Univ, Test Ctr, 48 Wenhui East Rd, Yangzhou 225009, Jiangsu, Peoples R China
来源：
ACTA VIROLOGICA | 2017年 / 61卷 / 04期
基金：
美国国家科学基金会;
关键词：
BoHV-1; calcium; calcium channel blocker; 2-AMINOETHOXYDIPHENYL BORATE 2-APB; RESPIRATORY-DISEASE; PHOSPHOLIPASE-C; VERAPAMIL; ENTRY; ACTIVATION; RELEASE; INHIBIT; CHANNELS; VIRUSES;
D O I：
10.4149/av_2017_412
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Calcium is one of the most prominent second messengers in eukaryotic cells. The involvement of calcium signaling in bovine herpesvirus 1 (BoHV-1) replication was not yet reported. In this study, we revealed that the L-type Ca2+ calcium channel blocker, Verapamil and store-operated calcium channel blocker, 2-aminoethyl diphenylborinate (2-APB) inhibited BoHV-1 replication in MDBK cells at the post-entry stages, and the Na+/Ca2+ exchanger inhibitor, N-arachidonoyl glycine exchanger (NAGly) interfered with the viral entry process. NAGly also effected the phosphorylation of PLC gamma-1 at Ser1248, which corroborated our previous findings, that PLC gamma-1 is important for BoHV-1 entry. Collectively, these results suggest that diverse calcium channels are employed by BoHV-1 for efficient replication.
引用
收藏
页码：487 / 491
页数：5
相关论文
共 26 条
[1]   Calcium signaling through phospholipase C activates dendritic cells to mature and is necessary for the activation and maturation of dendritic cells induced by diverse agonists [J].
Bagley, KC ;
Abdelwahab, SF ;
Tuskan, RG ;
Lewis, GK .
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 2004, 11 (01) :77-82
[2]   Calcium signalling: Dynamics, homeostasis and remodelling [J].
Berridge, MJ ;
Bootman, MD ;
Roderick, HL .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2003, 4 (07) :517-529
[3]   2-Aminoethoxydiphenyl borate (2-APB) is a reliable blocker of store-operated Ca2+ entry but an inconsistent inhibitor of InsP3-induced Ca2+ release [J].
Bootman, MD ;
Collins, TJ ;
Mackenzie, L ;
Roderick, HL ;
Berridge, MJ ;
Peppiatt, CM .
FASEB JOURNAL, 2002, 16 (10) :1145-1150
[4]   Release of Intracellular Calcium Stores Facilitates Coxsackievirus Entry into Polarized Endothelial Cells [J].
Bozym, Rebecca A. ;
Morosky, Stefanie A. ;
Kim, Kwang S. ;
Cherry, Sara ;
Coyne, Carolyn B. .
PLOS PATHOGENS, 2010, 6 (10)
[5]   Herpes simplex virus triggers activation of calcium-signaling pathways [J].
Cheshenko, N ;
Del Rosario, B ;
Woda, C ;
Marcellino, D ;
Satlin, LM ;
Herold, BC .
JOURNAL OF CELL BIOLOGY, 2003, 163 (02) :283-293
[6]   Rubella Virus: First Calcium-Requiring Viral Fusion Protein [J].
Dube, Mathieu ;
Rey, Felix A. ;
Kielian, Margaret .
PLOS PATHOGENS, 2014, 10 (12)
[7]   A Ca2+-dependent signalling circuit regulates influenza A virus internalization and infection [J].
Fujioka, Yoichiro ;
Tsuda, Masumi ;
Nanbo, Asuka ;
Hattori, Tomoe ;
Sasaki, Junko ;
Sasaki, Takehiko ;
Miyazaki, Tadaaki ;
Ohba, Yusuke .
NATURE COMMUNICATIONS, 2013, 4
[8]   Detection and characterization of viruses as field and vaccine strains in feedlot cattle with bovine respiratory disease [J].
Fulton, R. W. ;
d'Offay, J. M. ;
Landis, C. ;
Miles, D. G. ;
Smith, R. A. ;
Saliki, J. T. ;
Ridpath, J. F. ;
Confer, A. W. ;
Neill, J. D. ;
Eberle, R. ;
Clement, T. J. ;
Chase, C. C. L. ;
Burge, L. J. ;
Payton, M. E. .
VACCINE, 2016, 34 (30) :3478-3492
[9]   Ion transport blockers inhibit human rhinovirus 2 release [J].
Gazina, EV ;
Harrison, DN ;
Jefferies, M ;
Tan, H ;
Williams, D ;
Anderson, DA ;
Petrou, S .
ANTIVIRAL RESEARCH, 2005, 67 (02) :98-106
[10]   The clinically approved drugs amiodarone, dronedarone and verapamil inhibit filovirus cell entry [J].
Gehring, Gerrit ;
Rohrmann, Katrin ;
Atenchong, Nkacheh ;
Mittler, Eva ;
Becker, Stephan ;
Dahlmann, Franziska ;
Poehlmann, Stefan ;
Vondran, Florian W. R. ;
David, Sascha ;
Manns, Michael P. ;
Ciesek, Sandra ;
von Hahn, Thomas .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2014, 69 (08) :2123-2131
123