Detection of 1-O-malylglucose:: Pelargonidin 3-O-glucose-6"-O-malyltransferase activity in carnation (Dianthus caryophyllus)

被引：26

作者：

Abe, Yutaka ^{[1
]}

Tera, Masayuki ^{[1
]}

Sasaki, Nobuhiro ^{[1
]}

Okamura, Masachika ^{[2
]}

Umemoto, Naoyuki ^{[3
]}

Momose, Masaki ^{[3
]}

Kawahara, Nobuo ^{[4
]}

Kamakura, Hiroyuki

Goda, Yukihiro ^{[4
]}

Nagasawa, Kazuo ^{[1
]}

Ozeki, Yoshihiro ^{[1
]}

机构：

[1] Tokyo Univ Agr & Technol, Fac Engn, Dept Biotechnol & Life Sci, Koganei, Tokyo 1848588, Japan

[2] Kirin Agribio Co Ltd, Plant Res Ctr, Sakura, Tochigi 3291414, Japan

[3] Kirin Holdings Co Ltd, Cent Labs Frontier Technol, Sakura, Tochigi 3291414, Japan

[4] Natl Inst Hlth & Sci, Setagaya Ku, Tokyo 1588501, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 373卷 / 04期

关键词：

acyltransferase; anthocyanin; carnation; malic acid; Dianthus caryophyllus;

D O I：

10.1016/j.bbrc.2008.04.153

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Carnations have anthocyanins acylated with malate. Although anthocyanin acyltransferases have been reported in several plant species, anthocyanin malyltransferase (AMalT) activity in carnation has not been identified. Here, an acyl donor substance of AMalT, 1-O-beta-D-malylglucose, was extracted and partially purified from the petals of carnation. This was synthesized chemically to analyze AMalT activity in a crude extract from carnation. Changes in the AMalT activity showed close correlation to the accumulation of pelargonidin 3-malylglucoside (Pet 3-malGlc) during the development of red petals of carnation, but neither AMalT activity nor Pet 3-malGlc accumulation was detectable in roots, stems and leaves. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：473 / 477

页数：5

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