Genome-Wide Characterization and Comparative Analysis of MYB Transcription Factors in Ganoderma Species

Numerous studies in plants have shown the vital roles of MYB transcription factors in signal transduction, developmental regulation, biotic/abiotic stress responses and secondary metabolism regulation. However, less is known about the functions ofMYBsinGanoderma. In this study, five medicinal macrofungi of genusGanodermawere subjected to a genome-wide comparative analysis ofMYBgenes. A total of 75MYBgenes were identified and classified into four types: 1R-MYBs (52), 2R-MYBs (19), 3R-MYBs (2) and 4R-MYBs (2). Gene structure analysis revealed varying exon numbers (3-14) and intron lengths (7-1058 bp), and noncanonical GC-AG introns were detected inG. lucidumandG. sinense. In a phylogenetic analysis, 69 out of 75MYBgenes were clustered into 15 subgroups, and both single-copy orthologous genes and duplicated genes were identified. The promoters of theMYBgenes harbored multiplecis-elements, and specific genes were co-expressed with theG. lucidum MYBgenes, indicating the potential roles of theseMYBgenes in stress response, development and metabolism. This comprehensive and systematic study of MYB family members provides a reference and solid foundation for further functional analysis ofMYBgenes inGanodermaspecies.