Heterogeneity of Genetic Damage in Cervical Nuclei and Lymphocytes in Women with Different Levels of Dysplasia and Cancer-Associated Risk Factors

被引：4

作者：

Alvarez-Moya, Carlos ^{[1
]}

Reynoso-Silva, Monica ^{[1
]}

Canales-Aguirre, Alejandro A. ^{[2
]}

Chavez-Chavez, Jose O. ^{[3
]}

Castaneda-Vazquez, Hugo ^{[4
]}

Feria-Velasco, Alfredo I. ^{[1
]}

机构：

[1] Univ Guadalajara, Cellular & Mol Biol Dept, Guadalajara 44100, Jalisco, Mexico

[2] CIATEJ, Unit Med & Pharmaceut Biotechnol, Guadalajara 44270, Jalisco, Mexico

[3] Inst Cancerol Jalisci, Dysplasias Unit, Guadalajara 44100, Jalisco, Mexico

[4] Univ Guadalajara, Mastitis & Mol Diag, Guadalajara 44100, Jalisco, Mexico

来源：

BIOMED RESEARCH INTERNATIONAL | 2015年 / 2015卷

关键词：

DOUBLE-STRAND BREAKS; DNA-DAMAGE; COMET ASSAY; HUMAN-PAPILLOMAVIRUS; CELL-CYCLE; AGE; MICRONUCLEI; BLEOMYCIN; REPAIR; SMEARS;

D O I：

10.1155/2015/293408

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The comet assay can be used to assess genetic damage, but heterogeneity in the length of the tails is frequently observed. The aims of this study were to evaluate genetic damage and heterogeneity in the cervical nuclei and lymphocytes from patients with different levels of dysplasia and to determine the risk factors associated with the development of cervical cancer. The study included 97 females who presented with different levels of dysplasia. A comet assay was performed in peripheral blood lymphocytes and cervical epithelial cells. Significant genetic damage (P <= 0.05) was observed only in patients diagnosed with nuclei cervical from dysplasia III (NCDIII) and lymphocytes from dysplasia I (LDI). However, the standard deviations of the tail lengths in the cervical nuclei and lymphocytes from patients with dysplasia I were significantly different (P <= 0.0001) from the standard deviations of the tail lengths in the nuclei cervical and lymphocytes from patients with DII and DIII (NCDII, NCDIII and LDII, LDIII), indicating a high heterogeneity in tail length. Results suggest that genetic damage could be widely present but only manifested as increased tail length in certain cell populations. This heterogeneity could obscure the statistical significance of the genetic damage.

引用

页数：6

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