Identification of guitarfish species Rhinobatos percellens, R. horkelli, and Zapteryx brevirostris (Chondrichthyes) using mitochondrial genes and RFLP technique

被引：8

作者：

Mariguela, T. C. ^{[1
]}

De-Franco, B. ^{[1
]}

Almeida, T. V. V. ^{[2
]}

Mendonca, F. F. ^{[1
]}

Gadig, O. B. F. ^{[2
]}

Foresti, F. ^{[1
]}

Oliveira, C. ^{[1
]}

机构：

[1] Univ Estadual Paulista, Lab Biol & Genet Peixes, Dept Morfol, Inst Biociencias, BR-18601800 Botucatu, SP, Brazil

[2] Univ Estadual Paulista, UNESP, Sao Vicente, SP, Brazil

来源：

CONSERVATION GENETICS RESOURCES | 2009年 / 1卷 / 01期

基金：

巴西圣保罗研究基金会;

关键词：

Conservation genetics; Mitochondrial genes; Commercial species; Rays; Species identification; DNA; SHARK; PCR;

D O I：

10.1007/s12686-009-9091-y

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

The RFLP represents a fast and non-expensive tool to access species identification and can be used by fishery and law enforcement authorities to gather data from legal and illegal ray fisheries. In the present study, partial sequences of 16S and COI mitochondrial genes were submitted to RFLP analysis and the results showed that there is a high interspecific variability and no intraspecific polymorphism, making them an useful marker for guitarfish identification. All samples of Rhinobatidae: Rhinobatos percellens, R. horkelli, and Zapteryx brevirostris were positively identified. The main contribution of these forensic identification techniques is the possibility of evaluating population genetics of species status in order to improve conservation plans involving these species.

引用

页码：393 / 396

页数：4

共 17 条

[1] Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques [J].

Aljanabi, SM ;

Martinez, I .

NUCLEIC ACIDS RESEARCH, 1997, 25 (22) :4692-4693

[2]

Camhi M, 1998, 20 IUCN

[3]

COMPAGNO LJV, 1999, SHARKS SKATES RAYS B, P470

[4] Forensic identification of the guitarfish species Rhinobatos horkelli, R-percellens and Zapteryx brevirostris using multiplex-PCR [J].

De-Franco, B. ;

Mendonca, F. F. ;

Hashimoto, D. T. ;

Porto-Foresti, F. ;

Oliveira, C. ;

Foresti, F. .

MOLECULAR ECOLOGY RESOURCES, 2010, 10 (01) :197-199

[5]

Greig TW, 2005, FISH B-NOAA, V103, P516

[6]

Hall TA., 1999, Nucleic Acids Symp, V41, P95, DOI [10.1021/bk-1999-0734.ch008, DOI 10.14601/PHYTOPATHOLMEDITERR-14998U1.29]

[7] Shark fishing in fin soup [J].

Hoelzel, A. R. .

CONSERVATION GENETICS, 2001, 2 (01) :69-72

[8] Identification of shark and ray fins using DNA barcoding [J].

Holmes, Bronwyn H. ;

Steinke, Dirk ;

Ward, Robert D. .

FISHERIES RESEARCH, 2009, 95 (2-3) :280-288

[9] Identification of the shark species Rhizoprionodon lalandii and R-porosus (Elasmobranchii, Carcharhinidae) by multiplex PCR and PCR-RFLP techniques [J].

Mendonca, F. F. ;

Hashimoto, D. T. ;

Porto-Foresti, F. ;

Oliveira, C. ;

Gadig, O. B. F. ;

Foresti, F. .

MOLECULAR ECOLOGY RESOURCES, 2009, 9 (03) :771-773

[10]

Miranda L.D., 2003, Frente Maritimo, V19, P217

← 1 2 →