High-Throughput Sequencing Reveals Circulating miRNAs as Potential Biomarkers of Kidney Damage in Patients with Systemic Lupus Erythematosus

被引：45

作者：

Navarro-Quiroz, Elkin ^{[1
]}

Pacheco-Lugo, Lisandro ^{[1
]}

Lorenzi, Hernan ^{[2
]}

Diaz-Olmos, Yirys ^{[1
]}

Almendrales, Lisneth ^{[1
]}

Rico, Edwin ^{[1
]}

Navarro, Roberto ^{[3
]}

Espana-Puccini, Pierine ^{[4
]}

Iglesias, Antonio ^{[5
]}

Egea, Eduardo ^{[6
]}

Aroca, Gustavo ^{[1
,7
]}

机构：

[1] Univ Simon Bolivar, Grp Nefrol, Barranquilla, Colombia

[2] J Craig Venter Inst, Dept Infect Dis, Rockville, MD USA

[3] Univ Cooperat Colombia, Grp Genet, Santa Marta, Colombia

[4] Monomeros, Barranquilla, Colombia

[5] Univ Nacl Colombia, Unidad Reumatol, Bogota, Colombia

[6] Univ Norte, Grp Invest Inmunol & Biol Mol, Barranquilla, Colombia

[7] Clin Costa, Barranquilla, Colombia

来源：

PLOS ONE | 2016年 / 11卷 / 11期

关键词：

MICRORNA EXPRESSION; NEPHRITIS; CELLS; GLOMERULONEPHRITIS; MECHANISMS; DISEASE; RNA;

D O I：

10.1371/journal.pone.0166202

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Renal involvement is one of the most severe manifestations of systemic lupus erythematosus (SLE). Renal biopsy is the gold standard when it comes to knowing whether a patient has lupus nephritis, and the degree of renal disease present. However, the biopsy has various complications, bleeding being the most common. Therefore, the development of alternative, non-invasive diagnostic tests for kidney disease in patients with SLE is a priority. Micro RNAs (miRNAs) are differentially expressed in various tissues, and changes in their expression have been associated with several pathological processes. The aim of this study was to identify changes in the abundance of miRNAs in plasma samples from patients with lupus nephritis that could potentially allow the diagnosis of renal damage in SLE patients. This is an observational case-control cross-sectional study, in which we characterized the differential abundance profiles of miRNAs among patients with different degrees of lupus compared with SLE patients without renal involvement and healthy control individuals. We found 89 miRNAs with changes in their abundance between lupus nephritis patients and healthy controls, and 17 miRNAs that showed significant variations between SLE patients with or without renal involvement. Validation for qPCR of a group of miRNAs on additional samples from lupus patients with or without nephritis, and from healthy individuals, showed that five miRNAs presented an average detection sensitivity of 97%, a specificity of 70.3%, a positive predictive value of 82.5%, a negative predictive value of 96% and a diagnosis efficiency of 87.9%. These results strongly suggest that miR-221- 5p, miR-380-3p, miR-556-5p, miR-758-3p and miR-3074-3p are potential diagnostic biomarkers of lupus nephritis in patients with SLE. The observed differential pattern of miRNA abundance may have functional implications in the pathophysiology of SLE renal damage.

引用

页数：12