Optimization of submerged fermentation medium for citrinin-free monascin production by Monascus

被引：21

作者：

Chen, Di ^{[1
]}

Xue, Yuan ^{[1
]}

Chen, Mianhua ^{[1
]}

Li, Zhenjing ^{[1
]}

Wang, Changlu ^{[1
]}

机构：

[1] Tianjin Univ Sci & Technol, Sch Food Engn & Biotechnol, Key Lab Food Nutr & Safety, Minist Educ, Tianjin, Peoples R China

来源：

PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY | 2016年 / 46卷 / 08期

基金：

中国国家自然科学基金;

关键词：

Ammonium nitrate; carbon source; citrinin; mineral salts; monascin; nitrogen source; SOLID-STATE FERMENTATION; PIGMENT PRODUCTION; NITROGEN-SOURCES; PURPUREUS; GROWTH; CULTURE; BIOSYNTHESIS; METABOLITES; ANKAFLAVIN; SUGARS;

D O I：

10.1080/10826068.2015.1135461

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Microbial fermentation of citrinin-free Monascus pigments is in favor in the development of food industry. This study investigated the influences of carbon source, nitrogen source, and mineral salts on the cell growth, monascin (MS), and citrinin (CT) production in Monascus M9. A culture medium composition was established for maximizing the production of citrinin-free MS in submerged culture, as follows: 50g/L Japonica rice powder, 20g/L NH4NO3, 3g/L NaNO3, 1.5g/L KH2PO4, 1g/L MgSO(4)7H(2)O, 0.2g/L MnSO4. Under these conditions, no CT was detectable by high performance liquid chromatography. The yield of MS reached 14.11mg/g, improving approximately 30% compared with before optimization.

引用

页码：772 / 779

页数：8

共 22 条

[1] Effect of light on growth, pigment production and culture morphology of Monascus purpureus in solid-state fermentation [J].

Babitha, Sumathy ;

Carvahlo, Julio C. ;

Soccol, Carlos R. ;

Pandey, Ashok .

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2008, 24 (11) :2671-2675

[2] Solid-state fermentation for the production of Monascus pigments from jackfruit seed [J].

Babitha, Sumathy ;

Soccol, Carlos R. ;

Pandey, Ashok .

BIORESOURCE TECHNOLOGY, 2007, 98 (08) :1554-1560

[3] PRODUCTION OF CITRININ BY VARIOUS SPECIES OF MONASCUS [J].

BLANC, PJ ;

LORET, MO ;

GOMA, G .

BIOTECHNOLOGY LETTERS, 1995, 17 (03) :291-294

[4] EFFECT OF DIFFERENT NITROGEN-SOURCES ON PIGMENT PRODUCTION AND SPORULATION OF MONASCUS SPECIES IN SUBMERGED, SHAKEN CULTURE [J].

CARELS, M ;

SHEPHERD, D .

CANADIAN JOURNAL OF MICROBIOLOGY, 1977, 23 (10) :1360-1372

[5] Exploring the Distribution of Citrinin Biosynthesis Related Genes among Monascus Species [J].

Chen, Yi-Pei ;

Tseng, Ching-Ping ;

Chien, I-Ling ;

Wang, Wei-Yi ;

Liaw, Li-Ling ;

Yuan, Gwo-Fang .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2008, 56 (24) :11767-11772

[6] Protective Effect of Monascus-Fermented Red Mold Rice against Alcoholic Liver Disease by Attenuating Oxidative Stress and Inflammatory Response [J].

Cheng, Chin-fu ;

Pan, Tzu-Ming .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2011, 59 (18) :9950-9957

[7] A COLORIMETRIC METHOD FOR THE DETERMINATION OF SUGARS [J].

DUBOIS, M ;

GILLES, K ;

HAMILTON, JK ;

REBERS, PA ;

SMITH, F .

NATURE, 1951, 168 (4265) :167-167

[8] Elimination of the mycotoxin citrinin production in the industrial important strain Monascus purpureus SM001 [J].

Jia, Xiao Qin ;

Xu, Zhi Nan ;

Zhou, Li Ping ;

Sung, Chang Keun .

METABOLIC ENGINEERING, 2010, 12 (01) :1-7

[9] The Effect of Monascus Secondary Polyketide Metabolites, Monascin and Ankaflavin, on Adipogenesis and Lipolysis Activity in 3T3-L1 [J].

Jou, Pey-Chyi ;

Ho, Bing-Ying ;

Hsu, Ya-Wen ;

Pan, Tzu-Ming .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2010, 58 (24) :12703-12709

[10] Secondary metabolites of the fungus Monascus: A review [J].

Juzlova, P ;

Martinkova, L ;

Kren, V .

JOURNAL OF INDUSTRIAL MICROBIOLOGY, 1996, 16 (03) :163-170

← 1 2 3 →