The Role of EGF Receptor Ubiquitination in Regulating Its Intracellular Traffic

被引：80

作者：

Eden, Emily R. ^{[2
]}

Huang, Fangtian ^{[1
]}

Sorkin, Alexander ^{[1
]}

Futter, Clare E. ^{[2
]}

机构：

[1] Univ Pittsburgh, Sch Med, Dept Cell Biol & Physiol, Pittsburgh, PA 15261 USA

[2] UCL, Inst Ophthalmol, London EC1V 9EL, England

来源：

TRAFFIC | 2012年 / 13卷 / 02期

基金：

英国惠康基金;

关键词：

EGF receptor; intraluminal vesicles; multivesicular bodies; recycling; ubiquitination; GROWTH-FACTOR-RECEPTOR; CLATHRIN-COATED VESICLES; MULTIVESICULAR ENDOSOMES; INWARD VESICULATION; ENDOCYTOSIS; MECHANISM; INTERNALIZATION; BIOGENESIS; COMPLEXES; PATHWAY;

D O I：

10.1111/j.1600-0854.2011.01305.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Progression of activated EGF receptor (EGFR) through the endocytic pathway regulates EGFR signaling. Here we show that a non-ubiquitinated EGFR mutant, unable to bind the endosomal-sorting complex required for transport (ESCRT) component, Hrs, is not efficiently targeted onto intraluminal vesicles (ILVs) of multivesicular endosomes/bodies (MVBs). Moreover, ubiquitination and ESCRT engagement of activated EGFR are required for EGF-stimulated ILV formation. Non-ubiquitinated EGFRs enter clathrin-coated tubules emanating from MVBs and show enhanced recycling to the plasma membrane, compared to wild-type EGFR.

引用

页码：329 / 337

页数：9

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