The Role of EGF Receptor Ubiquitination in Regulating Its Intracellular Traffic

被引：79
作者：
Eden, Emily R. [2 ]
Huang, Fangtian [1 ]
Sorkin, Alexander [1 ]
Futter, Clare E. [2 ]
机构：
[1] Univ Pittsburgh, Sch Med, Dept Cell Biol & Physiol, Pittsburgh, PA 15261 USA
[2] UCL, Inst Ophthalmol, London EC1V 9EL, England
来源：
TRAFFIC | 2012年 / 13卷 / 02期
基金：
英国惠康基金;
关键词：
EGF receptor; intraluminal vesicles; multivesicular bodies; recycling; ubiquitination; GROWTH-FACTOR-RECEPTOR; CLATHRIN-COATED VESICLES; MULTIVESICULAR ENDOSOMES; INWARD VESICULATION; ENDOCYTOSIS; MECHANISM; INTERNALIZATION; BIOGENESIS; COMPLEXES; PATHWAY;
D O I：
10.1111/j.1600-0854.2011.01305.x
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Progression of activated EGF receptor (EGFR) through the endocytic pathway regulates EGFR signaling. Here we show that a non-ubiquitinated EGFR mutant, unable to bind the endosomal-sorting complex required for transport (ESCRT) component, Hrs, is not efficiently targeted onto intraluminal vesicles (ILVs) of multivesicular endosomes/bodies (MVBs). Moreover, ubiquitination and ESCRT engagement of activated EGFR are required for EGF-stimulated ILV formation. Non-ubiquitinated EGFRs enter clathrin-coated tubules emanating from MVBs and show enhanced recycling to the plasma membrane, compared to wild-type EGFR.
引用
收藏
页码：329 / 337
页数：9
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