Identification at strain level of Rhizoctonia solani AG4 isolates by direct sequence of asymmetric PCR products of the ITS regions

被引：84

作者：

Boysen, M ^{[1
]}

Borja, M ^{[1
]}

delMoral, C ^{[1
]}

Salazar, O ^{[1
]}

Rubio, V ^{[1
]}

机构：

[1] UNIV AUTONOMA MADRID,CSIC,CTR NACL BIOTECNOL,E-28049 MADRID,SPAIN

来源：

CURRENT GENETICS | 1996年 / 29卷 / 02期

关键词：

Rhizoctonia solani; ITS sequences; asymmetric PCR; phylogenetic tree;

D O I：

10.1007/BF02221582

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The relatedness of nine isolates of Rhizoctonia solani, belonging to anastomosis group (AG) 4, and one isolate of AG1 was determined by comparative sequence analysis based on direct sequencing of PCR-amplified ribosomal DNA [the internal transcribed spacer (ITS) region and the 5.8s ribosomal DNA]. The 5.8s rDNA is completely conserved, but both ITS regions show variation among strains. AG1 was an outgroup based on anastomosis ability and RFLP analyses. Phylogenetic analyses based on the ITS sequences suggest that the analyzed AG4 strains can be divided into three groups that correlate with habitat and virulence.

引用

页码：174 / 181

页数：8

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