Efficient gene delivery by a peptide derived from a monoclonal anti-DNA antibody

被引：19
作者：
Avrameas, A
Ternynck, T
Gasmi, L
Buttin, G
机构：
[1] Inst Pasteur, Unite Genet Somat, URA CNRS 1960, F-75724 Paris, France
[2] Inst Pasteur, Dept Immunol, Unite Immunocytochim, F-75724 Paris, France
来源：
BIOCONJUGATE CHEMISTRY | 1999年 / 10卷 / 01期
关键词：
D O I：
10.1021/bc980081c
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We recently reported that translocating murine polyreactive anti-DNA antibodies can be used as vectors for the transfer of macromolecules into cells growing in culture. We show here that two such monoclonal antibodies (J20.8 and F4.1) conjugated to polylysine with a high (93) but not a low (19) number of lysine residues can transfer genes in the presence of serum. A 30 amino acid long peptide, VAYISRGGVSTYYSDTVKGRFTRQKYNKRA (peptide P3), corresponding to joined heavy-chain complementary-determining regions 2 and 3 of F4.1 antibody and carrying 19 lysine :residues at its N-terminal, was found to be an efficient vector for the transfection of the luciferase gene into 3T3 and CCL39 cells in the presence of serum. Addition of 0.23 M glycerol during transfection considerably enhanced gene delivery. These results show that conjugation of a short polylysine tail converted a spontaneously internalizing peptide into a potent nontoxic plasmid vector.
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页码：87 / 93
页数：7
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