Structural Evidence for Standard-Mechanism Inhibition in Metallopeptidases from a Complex Poised to Resynthesize a Peptide Bond

被引：23

作者：

Arolas, Joan L. ^{[1
]}

Botelho, Tiago O. ^{[1
]}

Vilcinskas, Andreas ^{[2
]}

Xavier Gomis-Rueth, F. ^{[1
]}

机构：

[1] CSIC, Mol Biol Inst Barcelona, Proteolysis Lab, E-08028 Barcelona, Spain

[2] Univ Giessen, Inst Phytopathol & Angew Zool, D-35392 Giessen, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2011年 / 50卷 / 44期

关键词：

enzymes; metallopeptidases; protein inhibitors; structure elucidation; thermolysin; METALLOPROTEINASE INHIBITOR; REACTIVE-SITE; THERMOLYSIN;

D O I：

10.1002/anie.201103262

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

It goes both ways: An unprecedented mechanism of metalloendopeptidase inhibition has been identified for the insect metalloproteinase inhibitor, which is both cleaved and rejoined at bond Asn56-Ile57 by thermolysin under appropriate conditions. A two-product complex is formed after hydrolysis and, simultaneously, a Michaelis complex is poised for synthesis of a peptide bond (see crystal structure). Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：10357 / 10360

页数：4

共 18 条

[11] THE INFLUENCE OF WATER ON PROTEASE-CATALYZED PEPTIDE-SYNTHESIS IN ACETONITRILE WATER MIXTURES [J].

RESLOW, M ;

ADLERCREUTZ, P ;

MATTIASSON, B .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1988, 177 (02) :313-318

[12]

Seeram SS, 1997, J BIOCHEM-TOKYO, V122, P788

[13]

Seeram SS, 1997, J BIOCHEM-TOKYO, V121, P1088

[14]

Van Den Burg B., 2004, HDB PROTEOLYTIC ENZY, P374

[15] Effects of the entomopathogenic fungus Metarhizium anisopliae and its secondary metabolites on morphology and cytoskeleton of plasmatocytes isolated from the greater wax moth, Galleria mellonella [J].

Vilcinskas, A ;

Matha, V ;

Götz, P .

JOURNAL OF INSECT PHYSIOLOGY, 1997, 43 (12) :1149-1159

[16] THERMOLYSIN-CATALYZED PEPTIDE-BOND SYNTHESIS [J].

WAYNE, SI ;

FRUTON, JS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (11) :3241-3244

[17] Purification and characterization of an inducible metalloprotease inhibitor from the hemolymph of greater wax moth larvae, Galleria mellonella [J].

Wedde, M ;

Weise, C ;

Kopacek, P ;

Franke, P ;

Vilcinskas, A .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1998, 255 (03) :535-543

[18] The insect metalloproteinase inhibitor gene of the lepidopteran Galleria mellonella encodes two distinct inhibitors [J].

Wedde, Marianne ;

Weise, Christoph ;

Nuck, Rolf ;

Altincicek, Boran ;

Vilcinskas, Andreas .

BIOLOGICAL CHEMISTRY, 2007, 388 (01) :119-127

← 1 2 →