Structural Evidence for Standard-Mechanism Inhibition in Metallopeptidases from a Complex Poised to Resynthesize a Peptide Bond

被引：23

作者：

Arolas, Joan L. ^{[1
]}

Botelho, Tiago O. ^{[1
]}

Vilcinskas, Andreas ^{[2
]}

Xavier Gomis-Rueth, F. ^{[1
]}

机构：

[1] CSIC, Mol Biol Inst Barcelona, Proteolysis Lab, E-08028 Barcelona, Spain

[2] Univ Giessen, Inst Phytopathol & Angew Zool, D-35392 Giessen, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2011年 / 50卷 / 44期

关键词：

enzymes; metallopeptidases; protein inhibitors; structure elucidation; thermolysin; METALLOPROTEINASE INHIBITOR; REACTIVE-SITE; THERMOLYSIN;

D O I：

10.1002/anie.201103262

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

It goes both ways: An unprecedented mechanism of metalloendopeptidase inhibition has been identified for the insect metalloproteinase inhibitor, which is both cleaved and rejoined at bond Asn56-Ile57 by thermolysin under appropriate conditions. A two-product complex is formed after hydrolysis and, simultaneously, a Michaelis complex is poised for synthesis of a peptide bond (see crystal structure). Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：10357 / 10360

页数：4

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