A polymerase chain reaction-based screening method for transgenic Arabidopsis

被引：3

作者：

De Neef, V ^{[1
]}

Van Caeneghem, W ^{[1
]}

Marichal, M ^{[1
]}

Van Montagu, M ^{[1
]}

Van Der Straeten, D ^{[1
]}

机构：

[1] State Univ Ghent VIB, Genet Lab, Dept Genet, B-9000 Ghent, Belgium

来源：

GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING | 1999年 / 15卷 / 01期

关键词：

Arabidopsis; PCR; regenerant; screening; transgenic plant;

D O I：

10.1016/S1050-3862(98)00012-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and rapid PCR-based method for screening transformed Arabidopsis plants has been developed. Based on the quantity of chlorophyll present in a protoplast suspension, the optimal amount of template is calculated and a fragment of the transgene is amplified. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：1 / 4

页数：4

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