Role of Ca2+ for the mechanical properties of fibrillin

被引:22
作者
Eriksen, TA
Wright, DM
Purslow, PP
Duance, VC
机构
[1] Royal Vet & Agr Univ, Dept Dairy & Food Sci, DK-1958 Frederiksberg C, Denmark
[2] Cariff Univ, Connect Tissue Biol Labs, Cariff Sch Biosci, Cardiff, S Glam, Wales
来源
PROTEINS-STRUCTURE FUNCTION AND GENETICS | 2001年 / 45卷 / 01期
关键词
microfibrils; micro-mechanics; microneedle technique; calcium binding; epidermal growth factor-like domain;
D O I
10.1002/prot.1127
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Fibrillin-rich microfibrils are important structural elements widespread throughout connective tissues. Genetic defects identified in the Ca2+ binding sites of fibrillin have severe effects and in addition Ca2+ has a marked effect on the microfibrillar structure. We have studied the role of Ca2+ on the mechanical behavior of fibrillin-rich microfibrils using the micro-needle technique. We find that Ca2+-depletion results in a 50% decrease in rest length and reduces the stiffness of fibrillin-rich microfibrils. At high strain, irreversible damage occurs.. This behavior is consistent with Ca2+ stabilization of interact-ions between consecutive EGF-like domains and breakdown in the quaternary structure upon over-extension. (C) 2001 Wiley-Liss, Inc.
引用
收藏
页码:90 / 95
页数:6
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