Isolation and characterization of eleven polymorphic microsatellite markers for the earthworm Aporrectodea longa (Ude)

被引：7
作者：
Strunk, Heiko [1 ]
Hochkirch, Axel [2 ]
Veith, Michael [2 ]
Hankeln, Thomas [3 ]
Emmerling, Christoph [1 ]
机构：
[1] Univ Trier, Dept Soil Sci, D-54296 Trier, Germany
[2] Univ Trier, Dept Biogeog, D-54286 Trier, Germany
[3] GENterprise Genom, D-55128 Mainz, Germany
来源：
EUROPEAN JOURNAL OF SOIL BIOLOGY | 2012年 / 48卷
关键词：
Annelida; Microsatellite; Heterozygosity; Population genetics; Land-use change;
D O I：
10.1016/j.ejsobi.2011.11.004
中图分类号：
Q14 [生态学（生物生态学）];
学科分类号：
071012 ; 0713 ;
摘要：
Microsatellite markers are a promising tool for accurate estimation of population structure, dispersal and gene flow among populations. We isolated and characterized eleven new polymorphic microsatellite loci for the earthworm Aporrectodea longa (Ude), a widespread Palaearctic species. Polymorphism was tested in 31 individuals collected from one population and ranged from three to 15 alleles per locus. Observed heterozygosity ranged from 0.308 to 0.828, expected heterozygosity varied between 0.380 and 0.891. To the best of our knowledge, the presented microsatellite markers are the first for the species A. longa. Cross-species amplification tests indicated that the presented markers are not usable for other earthworm species. (C) 2011 Elsevier Masson SAS. All rights reserved.
引用
收藏
页码：56 / 58
页数：3
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