Isolation and characterization of eleven polymorphic microsatellite markers for the earthworm Aporrectodea longa (Ude)

被引：7
|
作者：
Strunk, Heiko [1 ]
Hochkirch, Axel [2 ]
Veith, Michael [2 ]
Hankeln, Thomas [3 ]
Emmerling, Christoph [1 ]
机构：
[1] Univ Trier, Dept Soil Sci, D-54296 Trier, Germany
[2] Univ Trier, Dept Biogeog, D-54286 Trier, Germany
[3] GENterprise Genom, D-55128 Mainz, Germany
来源：
EUROPEAN JOURNAL OF SOIL BIOLOGY | 2012年 / 48卷
关键词：
Annelida; Microsatellite; Heterozygosity; Population genetics; Land-use change;
D O I：
10.1016/j.ejsobi.2011.11.004
中图分类号：
Q14 [生态学（生物生态学）];
学科分类号：
071012 ; 0713 ;
摘要：
Microsatellite markers are a promising tool for accurate estimation of population structure, dispersal and gene flow among populations. We isolated and characterized eleven new polymorphic microsatellite loci for the earthworm Aporrectodea longa (Ude), a widespread Palaearctic species. Polymorphism was tested in 31 individuals collected from one population and ranged from three to 15 alleles per locus. Observed heterozygosity ranged from 0.308 to 0.828, expected heterozygosity varied between 0.380 and 0.891. To the best of our knowledge, the presented microsatellite markers are the first for the species A. longa. Cross-species amplification tests indicated that the presented markers are not usable for other earthworm species. (C) 2011 Elsevier Masson SAS. All rights reserved.
引用
收藏
页码：56 / 58
页数：3
相关论文
共 50 条
  • [21] Isolation and characterization of 20 novel polymorphic microsatellite markers for Pseudobagrus ussuriensis
    Chuankun Zhu
    Zhengjun Pan
    Hui Wang
    Nan Wu
    Guoliang Chang
    Huaiyu Ding
    Journal of Genetics, 2017, 96 : 35 - 38
  • [22] Isolation and characterization of eight polymorphic microsatellite markers from pink conch (Strombus gigas)
    Zamora-Bustillos, R.
    Rodriguez-Canul, R.
    De Leon, F. J. Garcia
    MOLECULAR ECOLOGY NOTES, 2007, 7 (04): : 597 - 599
  • [23] Isolation and characterization of polymorphic microsatellite markers from Japanese grenadier anchovy (Coilia nasus)
    Jin-quan Yang
    Xiao-du Zhou
    Dong Liu
    Zhi-zhi Liu
    Liang-jiang Wang
    Wen-qiao Tang
    Conservation Genetics Resources, 2012, 4 : 519 - 521
  • [24] Eleven highly polymorphic microsatellite markers for the lizard Anolis distichus
    Julienne Ng
    Susan L. Perkins
    Erica J. Dussmann
    Richard E. Glor
    Conservation Genetics Resources, 2009, 1 : 135 - 139
  • [25] Isolation and characterization of 12 polymorphic microsatellite markers for the frog Pelophylax hubeiensis
    Yan, J. -C.
    Zhu, F. -H.
    Wu, H. -L.
    GENETICS AND MOLECULAR RESEARCH, 2011, 10 (01): : 268 - 272
  • [26] Isolation and characterization of highly polymorphic microsatellite markers in Hypochaeris radicata (Asteraceae)
    Mix, C
    Arens, PFP
    Ouborg, NJ
    Smulders, MJM
    MOLECULAR ECOLOGY NOTES, 2004, 4 (04): : 656 - 658
  • [27] Eleven highly polymorphic microsatellite markers for the lizard Anolis distichus
    Ng, Julienne
    Perkins, Susan L.
    Dussmann, Erica J.
    Glor, Richard E.
    CONSERVATION GENETICS RESOURCES, 2009, 1 (01) : 135 - 139
  • [28] Isolation and characterization of 20 novel polymorphic microsatellite markers for Pseudobagrus ussuriensis
    Zhu, Chuankun
    Pan, Zhengjun
    Wang, Hui
    Wu, Nan
    Chang, Guoliang
    Ding, Huaiyu
    JOURNAL OF GENETICS, 2017, 96 : E35 - E38
  • [29] Isolation and characterization of eleven novel polymorphic microsatellite loci in the spiny softshell turtle (Apalone spinifera)
    Davy, Christina M.
    Conflitti, Ida M.
    Storisteanu, Daniel M. L.
    Murphy, Robert W.
    CONSERVATION GENETICS RESOURCES, 2012, 4 (03) : 759 - 761
  • [30] Isolation of polymorphic microsatellite markers in the tree sparrow (Passer montanus)
    Izumi, H.
    Hasegawa, O.
    Higashi, S.
    MOLECULAR ECOLOGY RESOURCES, 2009, 9 (01) : 245 - 247
12345