Viral colonization in exhaled breath condensate of lung cancer patients: Possible role of EBV and CMV

被引:11
作者
Carpagnano, Giovanna E. [1 ]
Lacedonia, Donato [1 ]
Natalicchio, Maria Iole [2 ]
Cotugno, Grazia [1 ]
Zoppo, Luigi [1 ]
Martinelli, Domenico [3 ]
Antonetti, Raffaele [2 ]
Foschino-Barbaro, Maria Pia [1 ]
机构
[1] Univ Foggia, Inst Resp Dis, Dept Med & Surg Sci, Foggia, Italy
[2] Riuniti Hosp, Mol Biol Lab, Foggia, Italy
[3] Univ Foggia, Sect Hyg, Dept Med Sci, Apulia Reg Epidemiol Observ, Foggia, Italy
关键词
CMV; EBV; lung cancer; EBC; EPSTEIN-BARR-VIRUS; LYMPHOEPITHELIOMA-LIKE CARCINOMA; NASOPHARYNGEAL CARCINOMA; CAPSID ANTIGEN; ANTIBODIES; NEOPLASMS; FIBROSIS; SMOKING; DNA;
D O I
10.1111/crj.12531
中图分类号
R56 [呼吸系及胸部疾病];
学科分类号
摘要
IntroductionToday, an increasing interest is being addressed to the viral etiology of lung tumors. As a consequence, research efforts are currently being directed to the identification of the new viruses involved in lung carcinogenesis toward which the screening programs could be directed. ObjectivesThe aim of this study was to investigate the airways colonization by the Epstein-Barr virus (EBV) and Citomegalovirus (CMV) in patients affected by lung cancer using, as a respiratory non-invasive sample, the exhaled breath condensate (EBC). MethodsAbout 70 lung-cancer patients and 40 controls were enrolled. All subjects underwent bronchial brushing and EBC collection. EBV-DNA and CMV-DNA were evaluated in both samples by real-time PCR assay. ResultsThey were able to detect EBV and CMV in the EBC. An increase of the EBV positivity in non-small cell lung cancer (NSCLC) patients compared with controls and of the CMV in advanced stages of lung cancer were observed. The association of the positivity of the cytology and the CMV test (in EBC or brushing) slightly increased the sensitivity of malignant diagnosis. ConclusionEBV and CMV resulted detectable in the EBC. In consideration of the potential involvement of these viruses in lung cancer, which was confirmed in this study, future studies in this direction were supported.
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页码:418 / 424
页数:7
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