Enantiomeric separation of triacylglycerols containing polyunsaturated fatty acids with 18 carbon atoms

被引：13

作者：

Rezanka, Tomas ^{[1
]}

Nedbalova, Linda ^{[2
]}

Sigler, Karel ^{[1
]}

机构：

[1] CAS, Inst Microbiol, Videnska 1083, Prague 14220, Czech Republic

[2] Charles Univ Prague, Dept Ecol, Fac Sci, Vinicna 7, CR-12844 Prague, Czech Republic

来源：

JOURNAL OF CHROMATOGRAPHY A | 2016年 / 1467卷

关键词：

Chiral triacylglycerols; Enantiomers; Regioisomers; Chiral chromatography; Tandem mass spectrometry; Alga; Haptophyte; Coccolithophora; PERFORMANCE LIQUID-CHROMATOGRAPHY; ALGA TRACHYDISCUS-MINUTUS; MASS-SPECTROMETRY; POSITIONAL ISOMERS; PHASE; TRIGLYCERIDES; OILS; MS;

D O I：

10.1016/j.chroma.2016.07.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Regioisomers and enantiomers of triacylglycerols (TAGs) containing any combination of stearidonic (18:4n-3) and octadecapentaenoic (18:5n-3) acids were prepared by organic synthesis. Gradient polar organic liquid chromatography/high resolution atmospheric pressure chemical ionization-tandem mass spectrometry (NARP-LC/HRMS2-APCI) and chiral liquid chromatography were used for the separation and identification of molecular species of these TAGs. Further, NARP-LC and chiral LC were used to separate natural mixtures of TAGs obtained from the haptophyte alga Coccolithophora sp. cultivated in a salinity range from 7.5 to 60 parts per thousand. The ratio of regioisomers and enantiomers was found to change with increasing salinity of the culture medium. This can be explained by variable activity of acyltransferases in cells exposed to salt stress. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：261 / 269

页数：9

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